Sequences from confirmation amplicon


Confirmation sequences for line 590C03 and hit At1g17840 are shown.
Line ID 590C03
Locus At1g17840 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:6145055
Primers used for PCR DC17 - 8474, show primer details
   
Sequence Name 15-K031246-022-590-C03C-8409
GenBank Accession LN492852 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>15-K031246-022-590-C03C-8409
ATTTACAATTGAATATATCCTGATCATGATAACTTTCATCTCCGGGACAATCTGTTACTT
CATGGTGGGTCTGCATCCAGGATTCACTCACTACCTCTTCTTTGTTCTCTGCCTCTATGC
AAGTGTCACCGTTGTGGAGAGCTTAATGATGGCAATTGCTAGTATTGTTCCCAACTTCCT
CATGGGTATCATCATCGGAGCTGGAATCCAGGTGATTTCTCCGACCACTTTCATCATAGA
CTTCTTAAAAGCAGAAGAGAAGCTCGTCCTAAATCTGGTTGTCTGTACTTTTGTTTCAGG
GGATCTTCATGCTGGTTTCTGGATTCTTCAGGCTTCCAAATGACATCCCAAAACCTTTCT
GGCGTTACCCAATGTCCTACATCAGCTTCCACTTCTGGGCTCTACAAGTAAATATTGTTG
AAACAAGAAAATGTTTCATATCAGCCCATCATCTTGTGATCAAAATAGCAGAGAGCTAAT
ACCGTCTTGTTTTACTTGTTCTCCAGGGTCAGTACCAGAATGATCTGAGAGGCTTGACGT
TTGACAGCCAAGGGAGTGCTTTCAAGATTCCGGGTGAATATGTTCTTGAGAACGTCTTTC
AGATCGACTTGCATCGATCAAAATGGATAAACCCTCAG
   
Sequence Name 16-K031246-022-590-C03C-DC17
GenBank Accession LN493319 [GenBank]
Sequencing primer gene specific primer, DC17: 5'-ACTGAGGTTAATCCATTTTGATCG-3'
Sequence (A. th genome BLAST matches underlined) 
>16-K031246-022-590-C03C-DC17
ATTCCCCGGAATCTTGAAAGCACTCCCTTGGCTGTCAAACGTCAAGCCTCTCAGATCATT
CTGGTACTGACCCTGGAGAACAAGTAAAACAAGACGGTATTAGCTCTCTGCTATTTTGAT
CACAAGATGATGGGCTGATATGAAACATTTTCTTGTTTCAACAATATTTACTTGTAGAGC
CCAGAAGTGGAAGCTGATGTAGGACATTGGGTAACGCCAGAAAGGTTTTGGGATGTCATT
TGGAAGCCTGAAGAATCCAGAAACCAGCATGAAGATCCCCTGAAACAAAAGTACAGACAA
CCAGATTTAGGACGAGCTTCTCTTCTGCTTTTAAGAAGTCTATGATGAAAGTGGTCGGAG
AAATCACCTGGATTCCAGCTCCGATGATGATACCCATGAGGAAGTTGGGAACAATACTAG
CAATTGCCATCATTAAGCTCTCCACAACGGTGACACTTGCATAGAGGCAGAGAACAAAGA
AGAGGTAGTGAGTGAATCCTGGATGCAGACCCACCATGAAGTAACAGATTGTCCCGGAGA
TGAAAGTTATCATGATCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTA
CATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATT
TTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTG
ATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCT
AATTCGGAAATCTTTATNTCGACGTGTCTACATTC

Last Updated on 06.12.2018 16:11