Sequences from confirmation amplicon


Confirmation sequences for line 713E10 and hit At1g17940 are shown.
Line ID 713E10
Locus At1g17940 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:6172133
Primers used for PCR 09yo - 8474, show primer details
   
Sequence Name 89-K120751-0022-713-E10C-09yo
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer gene specific primer, 09yo: 5'-CCAAAATCAGAAATCCTGGCTAGT-3'
Sequence (A. th genome BLAST matches underlined) 
>89-K120751-0022-713-E10C-09yo
TCCTCTCTGGGGAAATGTTGCTTAAGGTTTTTCCTTTTCTGTCAAGGATTTTGAATTATT
ATTATTTTTGGGGTTGAGCTCGATGAAGCATCACTTTCCATTACTCTTTTCTTACATTTG
AGCATAATTATTGGTGAGCCTTTACTCAAAATCTTCCCTTTTTAAGTCAAGTTGCTTTGC
AGCCTTTGAAATTCCTCGTTTTTTCTTTCCTATTTTCGTTATAACGTTTCATAGTGGAGA
AATGTGATTACAAACTGCAAAAAGTTTCATGCTTTCTCTGTGAACTCACATGCCTTTCTT
TTTTTGTTGGCTGATCAAGTTTGTATCTTTTTCTCTAATTTCTTGCAGAACTCGATTGGT
TTTGATAGCTTATAGCCAATCAAGGTGAGATAATCAGCAACCTCTTACTTGTTTATCAAA
CGTTGTTATTTGCTTGTTTGTTTCTGCTTGGAACTTGGTCTTAAAGTAAGATTATTGTTA
AGATTCGTGTAGATTATCTAGTCACGTTGAATTGTGAAGATGGTATAGTAATGGCAGATA
ACTCGTGAGCTGGGTTTTTACTTTATCCCTATGAATCTAGTGGAGGAGGACGGACTTGAG
CTGTTTTTTTCGGTTGGACATTAACTTTTATCAACTCCTTCTTATTGCATTTTTAGATGT
GGAATCATTCTCCTCTTTTCTTTTAGCTTGATCATTTTGTAGTCACAGATTGTTTTCTTG
TTTTTCAGGATATATTCAATTGTAAAGGGCTTCATGTCCGGGAAATCTACATGGATCAGC
AATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTATTA
   
Sequence Name 75-K120750-0022-713-E10C-8409
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>75-K120750-0022-713-E10C-8409
TTGAATATATCCTGAAAAACAGAAAACAATCTGTGACTACAAAATGATCAAGCTAAAAGA
AAAGAGGAGAATGATTCCACATCTAAAAATGCAATAAGAAGGAGTTGATAAAAGTTAATG
TCCAACCGAAAAAAACAGCTCAAGTCCGTCCTCCTCCACTAGATTCATAGGGATAAAGTA
AAAACCCAGCTCACGAGTTATCTGCCATTACTATACCATCTTCACAATTCAACGTGACTA
GATAATCTACACGAATCTTAACAATAATCTTACTTTAAGACCAAGTTCCAAGCAGAAACA
AACAAGCAAATAACAACGTTTGATAAACAAGTAAGAGGTTGCTGATTATCTCACCTTGAT
TGGCTATAAGCTATCAAAACCAATCGAGTTCTGCAAGAAATTAGAGAAAAAGATACAAAC
TTGATCAGCCAACAAAAAAAGAAAGGCATGTGAGTTCACAGAGAAAGCATGAAACTTTTT
GCAGTTTGTAATCACATTTCTCCACTATGAAACGTTATAACGAAAATAGGAAAGAAAAAA
CGAGGAATTTCAAAGGCTGCAAAGCAACTTGACTTAAAAAGGGAAGATTTTGAGTAAAGG
CTCACCAATAATTATGCTCAAATGTAAGAAAAGAGTAATGGAAAGTGATGCTTCATCGAG
CTCAACCCCAAAAATAATAATAATTCANAATCCTTGACAGAAAAGGAAAAACCTTAAGCA
ACATTTTCCCCAGAGAGGAATGAAGAANACACTATATACTACCAAAGCTGGACTAGCCA

Last Updated on 06.12.2018 16:11