Sequences from confirmation amplicon


Confirmation sequences for line 387H07 and hit At1g18160 are shown.
Line ID 387H07
Locus At1g18160 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:6249172
Primers used for PCR NK86 - 8474, show primer details
   
Sequence Name 46-K103393-0022-387-H07C-NK86
GenBank Accession LN505837 [GenBank]
Sequencing primer gene specific primer, NK86: 5'-AGGATCCTCCCTAGCACTTAACTC-3'
Sequence (A. th genome BLAST matches underlined) 
>46-K103393-0022-387-H07C-NK86
TTCTTCTTCACTTCTGGATCCTTGGAATTACTACTAGAACCCAAACCCTGCATCCCAGAT
TCCGACCCAACGGGACCACCATGTTCCATGGTAGTGTCATCCTCTTTGCTATTGGTGGCA
TTGGAAGAAGAAGGGCTTTTCCTGTGAGTAACAGAGCTTAACCAATTTGATAAACCCAAA
AAGGGTTTGAATTCAGAGTTATCTTGAGACCTCAATGAATCAGAAGGAGATGGTTCTGCA
TCACTAGACTTATTGCTTCTGTTAGAGACTGAACCTTCACCATCGTCTCGTTGATTGGTG
AATGGCTTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTA
TGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATG
TAGATTTCC
   
Sequence Name 36-K103394-0022-387-H07C-8409
GenBank Accession LN502191 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>36-K103394-0022-387-H07C-8409
GCCATTTACATTGAAGCCTTCACCAATCAACGAGACGATGGTGAAGGTTCAGTCTCTAAC
AGAAGCAATAAGTCTAGTGATGCAGAACCATCTCCTTCTGATTCATTGAGGTCTCAAGAT
AACTCTGAATTCAAACCCTTTTTGGGTTTATCAAATTGGTTAAGCTCTGTTACTCACAGG
AAAAGCCCTTCTTCTTCCAATGCCACCAATAGCAAAGAGGATGACACTACCATGGAACAT
GGTGGTCCCGTTGGGTCGGAATCTGGGATGCAGGGTTTGGGTTCTAGTAGTAATTCCNAG
GATCCNGAAGTTGAAGAAGAATATCAGATACNGTTGGCTCTNGAGTTAAGTGCTAGGGAG
GATC

Last Updated on 06.12.2018 16:11