Sequences from confirmation amplicon


Confirmation sequences for line 016D06 and hit At1g18190 are shown.
Line ID 016D06
Locus At1g18190 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:6260565
Primers used for PCR 02by - 8474, show primer details
   
Sequence Name 60-K113379-0022-016-D06C-02by
GenBank Accession LN510310 [GenBank]
Sequencing primer gene specific primer, 02by: 5'-CTTCCTCGTTAGACCATCAGAAAC-3'
Sequence (A. th genome BLAST matches underlined) 
>60-K113379-0022-016-D06C-02by
CTTTCCCATACAGAACTTCTTCTGGACTCACGACCATCCCTTTTTCCTACACTTGGTGAT
GTTTCCCTAGGGAGAGTCTCATTAGCCGATCTTCTGGAGTCATCCAGGGGAGGTGTCTTA
CCACTGACATCCTTCTCACTCTCTTTATGAGTTGAGTTCACAAATATATCCCGAGGAGCA
TTCCTATTTTTCATGTCAGCATCAGAAGGGCTAGACACTTCCTTGTCGCTTGGTTCCTTA
TTCGTCTGTTTTTGTGGCCTCCCTCTGGAATTAACCACATTACTACTACTACTCTTCTTT
CCATCAGATACCAAAGGATTTTTCCCCAAATTCCCATGTCTTTTTCCATCCTTCTTCAAT
CCCCGAATCGCAGATGTTCCACCAGGACTACGATTAGGAATGGCTTCAATTGTAAATGGC
TTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAA
GAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCG
   
Sequence Name 22-K113377-0022-016-D06C-8409
GenBank Accession LN496194 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>22-K113377-0022-016-D06C-8409
TCATTGAAGCCTTCCTAATCGTAGTCCTGGTGGAACATCTGCGATTCGGGGATTGAAGAA
GGATGGAAAAAGACATGGGAATTTGGGGAAAAATCCTTTGGTATCTGATGGAAAGAAGAG
TAGTAGTAGTAATGTGGTTAATTCCAGAGGGAGGCCACAAAAACAGACGAATAAGGAACC
AAGCGACAAGGAAGTGTCTAGCCCTTCTGATGCTGACATGAAAAATAGGAATGCTCCTCG
GGATATATTTGTGAACTCAACTCATAAAGAGAGTGAGAAGGATGTCAGTGGTAAGACACC
TCCCCTGGATGACTCCAGAAGATCGGCTAATGAGACTCTCCCTAGGGAAACATCACCAAG
TGTAGGAAAAAGGGATGGTCGTGAGTCCAGAAGAAGTTCTGTATGGGGAAAGCAGGTGAG
GGAAGAAGTCTCACAGAGCAATGTTTCTGATGGTCAACCGAGGAAG

Last Updated on 06.12.2018 16:11