Sequences from confirmation amplicon


Confirmation sequences for line 684A10 and hit At1g19240 are shown.
Line ID 684A10
Locus At1g19240 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:6650444
Primers used for PCR BM31 - 8474, show primer details
   
Sequence Name 36-K104374-0022-684-A10C-BM31
GenBank Accession LN502213 [GenBank]
Sequencing primer gene specific primer, BM31: 5'-TCACTCACTCACCCAAAATTCTTA-3'
Sequence (A. th genome BLAST matches underlined) 
>36-K104374-0022-684-A10C-BM31
CAATGATCTCTTTTGAACAATATTGAATAGAAGCAGCTTACCTGGGAGGTCTGGTTACCA
GAGTGCCGGATCCTGCGTCGTATGTAGTAGTCAAGTTGCCGGCGGCGGCTCGTTTGTCTT
GCATCAGATTATCCGCAAAATCAGATAGAGAAGACGACGAAGAAGAAGCTGCCGATTTCG
CTTCTTTCACCATCTTGCTGTAAAACTTCTCCAAACCCTCCATTTCTGGCCGATCAATTA
GGGTTTAGGGTTCAATGATGATTCCAGTCATGAAGGAAGCGATTGCTTCATGTCCGGGAA
ATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTAC
CAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGGGTTATTA
   
Sequence Name 52-K104375-0022-684-A10C-8409
GenBank Accession LN507808 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>52-K104375-0022-684-A10C-8409
GCATCGCTTCCTTCTGACTGGAATCATCATTGAACCCTAAACCCTAATTGATCGGCCAGA
AATGGAGGGTTTGGAGAAGTTTTACAGCAAGATGGTGAAAGAAGCGAAATCGGCAGCTTC
TTCTTCGTCGTCTTCTCTATCTGATTTTGCGGATAATCTGATGCAAGACAAACGAGCCGC
CGCCGGCAACTTGACTACTACATACGACGCAGGATCCGGCACTCTGGTAACCAGACCTCC
CAGGTAAGCTGCTTCTATTCAATATTTGTTCGAAAAGAGATCATTTTGCCAGTAAAGCTA
AGAACTTTTAAGAATTTTGGGTGAGTGAGTGA

Last Updated on 06.12.2018 16:11