Sequences from confirmation amplicon


Confirmation sequences for line 214G02 and hit At1g20900 are shown.
Line ID 214G02
Locus At1g20900 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:7272883
Primers used for PCR KT65 - 8474, show primer details
   
Sequence Name 84-K100817-0022-214-G02C-8409
GenBank Accession LN514391 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>84-K100817-0022-214-G02C-8409
GCCATCATACGAGAAGTCAAAACTCATGATTTTGAGGAAACAATAGCTAAAGCATTAAGG
TGTGATCGGTTACGCAAGCCGACAAAATATAGAAACAGACCAAGAACAGAACTAAATTAA
AGACGAAGCAAAAGATATAAAAAAGGTGACTTATGTTAAATGATCTCTCTCATGAAAGGT
AAGAGGAAACCCTAGTTAGGTAGATTAATTAGGGCAAGAGAAAGAAGTAGCTTCTTCTTT
GCATAGGAGTCGTATTTAGCGATTCTGATGTGCTATGGGAAAAAAAGGAGAGAGAAGCTT
GAGATTCATGTGCGACTCTGCAATAAATTTTTGGTCCTCTCATTGAACGAGGAAAGTAAA
AGCAAAGACATACACCGAGAATTTAGCGGAGAAACAGTCCCCTTTATTATATCATTAATA
TAATAATTATTATTTGAATAATATGTTCTTTTTTAAGTAAATTAATACTATTTATTACAT
ATACACAAA
   
Sequence Name 84-K100829-0022-214-G02C-KT65
GenBank Accession LN514392 [GenBank]
Sequencing primer gene specific primer, KT65: 5'-TTTTGTCGGCATTTGTGTATATGT-3'
Sequence (A. th genome BLAST matches underlined) 
>84-K100829-0022-214-G02C-KT65
TTAAAGACATATATTCAATAATAATTATTATATTAATGATATAATAAAGGGGACTGTTTC
TCCGCTAAATTCTCGGTGTATGTCTTTGCTTTTACTTTCCTCGTTCAATGAGAGGACCAA
AAATTTATTGCAGAGTCGCACATGAATCTCAAGCTTCTCTCTCCTTTTTTTCCCATAGCA
CATCAGAATCGCTAAATACGACTCCTATGCAAAGAAGAAGCTACTTCTTTCTCTTGCCCT
AATTAATCTACCTAACTAGGGTTTCCTCTTACCTTTCATGAGAGAGATCATTTAACATAA
GTCACCTTTTTTATATCTTTTGCTTCGTCTTTAATTTAGTTCTGTTCTTGGTCTGTTTCT
ATATNTTGTCGGCTTGCGTAACCGATCACACCTTAATGCTTTAGCTATTGTTTCCTCAAA
ATCATGAGTTTTGACTTCTCGTATGATGGCTTCATGTCCGGGAAATCTACATGGATCAGC
AATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATT
CANAAATGTAGATCTCC
Primers used for PCR KT66 - 8474, show primer details
   
Sequence Name 92-K100829-0022-214-G02C-KT66
GenBank Accession LN515171 [GenBank]
Sequencing primer gene specific primer, KT66: 5'-CACTCTCAACTATGTCAGCTCCAG-3'
Sequence (A. th genome BLAST matches underlined) 
>92-K100829-0022-214-G02C-KT66
TAGCGCGTTAGGGCTATCACGAGTTACTATGATCGGTGGCTTGGCTTTGTTCTTAGATCC
TGGTGGACGTCCACGTGGACGTTTTCCCGGTGCTGAGCTTGATGTATTCGGGTCTGAATC
GGGTCGACCCTGTTGATGATGATCTTTGTTTGAATGGTCAGATTCTCTTGAATCATCCGA
CGGTTGTTGTTGTTGTTGATGTTGCTGGTGCTGATGATGATGCTGGTCGATAAGATTGAT
CCCGCCCTGCGGTTGAAGCTGTTGGTGGTGAATCTCCGGTCTAAAGAGGTTATGGAAGTA
TCTAGAAGCTCCACCGCCTTGCTCGTAACCGCCTTCCATCGCCGCCGCATCTACTAATTC
CCTATCCCACCACTGCTTATTCGCCAGCTCANATCANTTTCTCGATTCTGGATCCTGCTC
GTTCATATATGGGTAGCTAGGTNAAGAAGAGCGTANAGAGAGAANAAGAANACTCATATC
AGGATATATTCAATTGTAAATGGCTTCATGTCCGGGGAATCTACATGGATCAGCAATGAG
TATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCANAAA
TGT

Last Updated on 06.12.2018 16:11