Sequences from confirmation amplicon


Confirmation sequences for line 038A12 and hit At1g22880 are shown.
Line ID 038A12
Locus At1g22880 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:8096744
Primers used for PCR MK76 - 8474, show primer details
   
Sequence Name 79-K101328-0022-038-A12C-8409
GenBank Accession LN513798 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>79-K101328-0022-038-A12C-8409
GAGCCATTTACAATTGAGTAATTGGGACAATAAATACGCCGGTGCCTATGTTCTTCTCTC
ACGAGTATGTCTCAATCCTTAAAATTCCTAGACATAACTTAACCATTTCCTTCAATGATT
TACAAACACATTTCTTTCGCAGCGAGCAGTACTAAACAAAGACAACAACTTTGAACTCTA
CAAGCAAGCAGCTGAGAATTTCATGTGTAAGATCCTTCCAAATTCTCCATCCTCGTCAAC
AAAGTACACTAAAGGTAAACTCAACACAGAGTTCTCTCCAAAAGCATTTCAATTACTCAA
ATACTAAAAGCTATAACTTTCAGGTGGACTGATGTACAAACTACCTCAGAGCAATCTACA
ATACGTGACATCAATAACATTCTTGCTCACCACCTACGCCAAATATATGAAATCCACAAA
ACAAACTTTCAACTGCGGAAACTCACTAATCGTCCCCAACGCACTGATNAACCTATCG
   
Sequence Name 79-K101334-0022-038-A12C-MK76
GenBank Accession LN513799 [GenBank]
Sequencing primer gene specific primer, MK76: 5'-TTCGATAGATTTATCAGTGCGTTG-3'
Sequence (A. th genome BLAST matches underlined) 
>79-K101334-0022-038-A12C-MK76
GTTGTTTTGTGGATTTCATATATTTGGCGTAGGTGGTGAGCAAGAATGTTATTGATGTCA
CGTATTGTAGATTGCTCTGAGGTAGTTTGTACATCAGTCCACCTGAAAGTTATAGCTTTT
AGTATTTGAGTAATTGAAATGCTTTTGGAGAGAACTCTGTGTTGAGTTTACCTTTAGTGT
ACTTTGTTGACGAGGATGGAGAATTTGGAAGGATCTTACACATGAAATTCTCAGCTGCTT
GCTTGTAGAGTTCAAAGTTGTTGTCTTTGTTTAGTACTGCTCGCTGCGAAAGAAATGTGT
TTGTAAATCATTGAAGGAAATGGTTAAGTTATGTCTAGGAATTTTAAGGATTGAGACATA
CTCGTGAGAGAAGAACATAGGCACCGGCGTATTTATTGTCCCAATTACTCAATTGTAAAT
GGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAA
AAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATATCCGC

Last Updated on 06.12.2018 16:11