Sequences from confirmation amplicon


Confirmation sequences for line 523B07 and hit At1g23080 are shown.
Line ID 523B07
Locus At1g23080 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:8181911
Primers used for PCR TE05 - 8474, show primer details
   
Sequence Name 03-K024844-022-523-B07C-8409
GenBank Accession LN487322 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>03-K024844-022-523-B07C-8409
CCGGACATGAAGCCATTTACAATTGAAAAAGAAATCTACATGTAGCCAATTACAATTGAA
TATGTCGGCACATATACATATTGTTTCTTATGAGTTTCCTCCACACCATTATCAATATCA
GCCGAGTCATCACACTCGCTGGTGGCATATGTTTTACCGGTACAGTTTCACCCGTTTCTA
TAGCTTCTTTAGGGTTTAGCTCTGCTGTGGAGTTACACCGAAGCTTGTGTAGTCCGTTAG
GCACTTCCTTTACCCTCTCCGACTCTTCTTCCCCGCCATAGTCCCCGTTCATCGGACCAG
CTACAAATTTAAGCAAATACAGTTTGAGATTTGTGTCCATATGTTACAAAAAAAAATACA
ATTTAAAAGTTAAAAACCTTTGTTTTCACCATTTTGAGTATGATCAGAGATCAACATTCG
AATCTCTTTTGCACCGCCTTGATCGGATTTTCCGACTTGTTCATTGGCTCCATTATCAAC
TTGAAGACCAGCTCGGTCCGAAACGGGTGATCCGTTGGATCCCCAAACAAACATATGAAG
CTCCTTAGCATCATTACTATTTGATTTTCCTACATGATGATGATTCTCTTTTGGAGCTTT
ACTACCGGTTTTATTACCGGTAGAAAACT
   
Sequence Name 04-K024844-022-523-B07C-TE05
GenBank Accession LN487761 [GenBank]
Sequencing primer gene specific primer, TE05: 5'-AGCTCCTAATCCGGAGTTTTCTAC-3'
Sequence (A. th genome BLAST matches underlined) 
>04-K024844-022-523-B07C-TE05
CGGTAGTAAAGCTCCAAAAGAGAATCATCATCATGTAGGAAAATCAAATAGTAATGATGC
TAAGGAGCTTCATATGTTTGTTTGGGGATCCAACGGATCACCCGTTTCGGACCGAGCTGG
TCTTCAAGTTGATAATGGAGCCAATGAACAAGTCGGAAAATCCGATCAAGGCGGTGCAAA
AGAGATTCGAATGTTGATCTCTGATCATACTCAAAATGGTGAAAACAAAGGTTTTTAACT
TTTAAATTGTATTTTTTTTTGTAACATATGGACACAAATCTCAAACTGTATTTGCTTAAA
TTTGTAGCTGGTCCGATGAACGGGGACTATGGCGGGGAAGAAGAGTCGGAGAGGGTAAAG
GAAGTGCCTAACGGACTACACAAGCTTCGGTGTAACTCCACAGCAGAGCTAAACCCTAAA
GAAGCTATAGAAACGGGTGAAACTGTACCGGTAAAACATATGCCACCAGCGAGTGTGATG
ACTCGGCTGATATTGATAATGGTGTGGAGGAAACTCATAAGAAACAATATGTATATGTGC
CGACATATTCAATTGTAATTGGCTACATGTAGATTTCTTTTTCAATTGTAAATGGCTTCA
TGTCCGGGAAATCTACATGGATCAGCAATGAGTATNGATGGTCAATATGGAGAAAAAGAA
AGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAA
ATGAAAGTACATTTTGATAAAAC

Last Updated on 06.12.2018 16:11