Sequences from confirmation amplicon


Confirmation sequences for line 196H08 and hit At1g23510 are shown.
Line ID 196H08
Locus At1g23510 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:8341451
Primers used for PCR 13uv - 8474, show primer details
   
Sequence Name 41-K117452-0022-196-H08C-13uv
GenBank Accession LN504236 [GenBank]
Sequencing primer gene specific primer, 13uv: 5'-AAACAAAAGAAGCCGAAGAGTACA-3'
Sequence (A. th genome BLAST matches underlined) 
>41-K117452-0022-196-H08C-13uv
TGGGTCGAAAGTGACATTGGACCAGATGTTTAAGCCTTCTTTTATTTTTAATCTGCCACC
GATTTCTTGGTACGTTCGTCGGATAACCATCAAGACCATGAAAACTCTGATCGAAGATCT
TCAAATTACAAGTGCCATGATCCGAGGTATTTCCAGGATATATTCAATTGTAAATGGCTT
CATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGA
AAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGATTATA
   
Sequence Name 18-K117451-0022-196-H08C-8409
GenBank Accession LN494486 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>18-K117451-0022-196-H08C-8409
TCCTGGAAATCCTCGGATCATGGCACTTGTAATTTGAAGATCTTCGATCAGAGTTTTCAT
GGTCTTGATGGTTATCCGACGAACGTACCAAGAAATCGGTGGCAGATTAAAAATAAAAGA
AGGCTTAAACATCTGGTCCAATGTCACTTTCGACCCAATCCTTCCTTGTCCGCGGCTACA
TCTTTCGTATTCTTCTGTACTCTTCGGCTTCTTTTGTTT
Primers used for PCR 09us - 8474, show primer details
   
Sequence Name 42-K117452-0022-196-H08C-09us
GenBank Accession LN504616 [GenBank]
Sequencing primer gene specific primer, 09us: 5'-CCTCATGAGAATCAATCCATTTTT-3'
Sequence (A. th genome BLAST matches underlined) 
>42-K117452-0022-196-H08C-09us
CTATTTTTGACAGGTACAATATACGGTAAAGAATAAGATGTTCATAAACATGTTTGTCAG
TATAAAGTGGAGCCGCTATACGTAGATTCAGAACGCTTGTGCAAACAAAAGAAGCCGAAG
AGTACAGAAGAATACGAAAGATGTAGCCGCGGACAAGGAAGGATTGGGTCGAAAGTGACA
TTGGACCAGATGTTTAAGCCTTCTTTTATTTTTAATCTGCCACCGATTTCTTGGTACGTT
CGTCGGATAACCATCAAGACCATGAAAACTCTGATCGAAGATCTTCAAATTACAAGTGCC
ATGATCCGAGGTATTTCCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCT
ACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAAT
TTTTTTTCAATTCAAAAATGTAGATGTCCGCA
   
Sequence Name 19-K117451-0022-196-H08C-oriL
GenBank Accession LN494937 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>19-K117451-0022-196-H08C-oriL
TCCTGGAAATCCTCGGATCATGGCACTTNTAATTTGAAGATCTTCGATCAGAGTTTTCAT
GGTCTTGATGGTTATCCGACGAACGTACCAAGAAATCGGTGGCAGATTAAAAATAAAAGA
AGGCTTAAACATCTGGTCCAATGTCACTTTCGACCCAATCCTTCCTTGTCCGCGGCTACA
TCTTTCGTATTCTTCTGTACTCTTCGGCTTCTTTTGTTTGCACAAGCGTTCTGAATCTAC
GTATAGCGGCTCCACTTTATACTGACAAACATGTTTATGAACATCTTATTCTTTACCGTA
TATTGTACCTGTCAAAAATAAGCAAGAGAAACCCAAATTAAATCTTTTCCAAGATTGAAA
AAAATGGATTGATCTTCATGAGG

Last Updated on 06.12.2018 16:11