Sequences from confirmation amplicon


Confirmation sequences for line 640C02 and hit At1g24270 are shown.
Line ID 640C02
Locus At1g24270 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:8608736
Primers used for PCR CH92 - 8474, show primer details
   
Sequence Name 28-K107246-0022-640-C02C-CH92
GenBank Accession LN498872 [GenBank]
Sequencing primer gene specific primer, CH92: 5'-TGAGGTTGTTCTTCCGATATCTCT-3'
Sequence (A. th genome BLAST matches underlined) 
>28-K107246-0022-640-C02C-CH92
TTTTTTGGAGTGTGGGAAGCGAGATCTCGTCCCTGGTGAGATGATCTTCAAAGGCTTCTA
TGATCTGGTTGATGAGCTCACGGTTAGACGAAGAATCCCATTTAGACCAGTAACTTGTGT
AGCAATCAAAACATTGACAGTCAAGCAGAGGAGCTTTATGGCTTCTCTTCTTCTTGCAAG
ATACGACGTTAGTGGTGTTGAGTGACCTGGTGATTAAGTAAGCAAGAACTTCCAGATCTT
CGGCGGAGAGAACCGAGACTAGAACTAGAATCGCACTCTGGAGAAGCTTAAAGACAGAAA
GAGAGTCATCTCCATTCGAAGAAGAAGAAGATGGAAGAGGAGGAGATGGGTGCACTTTAC
CTTTCTTCATTACTTTCATGGCTGATTTTGAGGAATCTTTCTTTTCTAGATAGAGAGAGA
GAGAGAAGCAGAGGAGAGAGTTTTCTTTGCTGCGATTATTGTTTCTTTCCTTAATGGCTT
TCGCAGATAAGAGGAAACAGAGTATAGAGCCTTTTTTCATGTTTATGTATTTCAATTGTA
AATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGA
GAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCACGNT
TATTA
   
Sequence Name 12-K107245-0022-640-C02C-8409
GenBank Accession LN491604 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>12-K107245-0022-640-C02C-8409
GCCATTTACAATTGAAATACATAAACATGAAAAAAGGCTCTATACTCTGTTTCCTCTTAT
CTGCGAAAGCCATTAAGGAAAGAAACAATAATCGCAGCAAAGAAAACTCTCTCCTCTGCT
TCTCTCTCTCTCTCTATCTAGAAAAGAAAGATTCCTCAAAATCAGCCATGAAAGTAATGA
AGAAAGGTAAAGTGCACCCATCTCCTCCTCTTCCATCTTCTTCTTCTTCGAATGGAGATG
ACTCTCTTTCTGTCTTTAAGCTTCTCCAGAGTGCGATTCTAGTTCTAGTCTCGGTTCTCT
CCGCCGAAGATCTGGAAGTTCTTGCTTACTTAATCACCAGGTCACTCAACACCACTAACG
TCGTATCTTGCAAGAAGAAGAGAAGCCATAAAGCTCCTCTGCTTGACTGTCAATGTTTTG
ATTGCTACACAAGTTACTGGTCTAAATGGGATTCTTCGTCTAACCGTGAGCTCATCAACC
AGATCATAGAAGCCTTTGAAGATCATCTCACCAGGGACGAGATCTCTGCTTCCCACACTT
CCAAGAAAAACAAGAAGAGAGCTAAGAAGATAGAGATATCGGAAGAACAACCTC

Last Updated on 06.12.2018 16:11