Sequences from confirmation amplicon


Confirmation sequences for line 879G09 and hit At1g25260 are shown.
Line ID 879G09
Locus At1g25260 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:8854181
Primers used for PCR DA72 - 8474, show primer details
   
Sequence Name 11-K107640-0022-879-G09C-DA72
GenBank Accession LN491143 [GenBank]
Sequencing primer gene specific primer, DA72: 5'-CTATGATCAGGCTCTGCTTCTCTT-3'
Sequence (A. th genome BLAST matches underlined) 
>11-K107640-0022-879-G09C-DA72
TTTATCTTAATACTATATTGGTACCACCTAAATGATTAGGTTGAACTGAAGGAAGGTCCT
CTAGAACAGTTCACACATGAGATGGAACCACTTCTTCGGAAGCTTGAAATGCCTGTACGA
CTAAACAAAGGTCTGTCGTCTCACCATCTTAATATTCCTCTTTCTCATACTCACTTTTGC
TATCTATATTAATCCAAAAACTTATTTCTTCTTCTTATTTTAATATGTAGGTACTGTGGA
GTTGGTCGCTGACTTTGTAGTTTGTGAAGAAGGAAAACAACTTTCGCCAAAATCAGCCCA
CATTTTGGTAATAAGACTTCTGTATACCAAAACCCTTTCCTTTTAATTTGTATGCTTTAT
TTCTTATACTTGTTTGTTTACTTTGCAGCGTCTATTGCGAATGAAGATGGCTACTTTCAA
GCTGAACTTGCTCTGCAGATGGAGCCCTAGTGACTTTGAGCTTTACAGAGAAGATCTGTC
AGAGCTTTACCGAGAAGATCTCTCATCACTATCAATTGTAAATGGCTTCATGTCCGGGAA
ATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTAC
CAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCACGGTTATTA
   
Sequence Name 27-K107641-0022-879-G09C-8409
GenBank Accession LN498417 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>27-K107641-0022-879-G09C-8409
CGGACTGAGCCATTTACATTGATAGTGATGAGAGATCTTCTCGGTAAAGCTCTGACAGAT
CTTCTCTGTAAAGCTCAAAGTCACTAGGGCTCCATCTGCAGAGCAAGTTCAGCTTGAAAG
TAGCCATCTTCATTCGCAATAGACGCTGCAAAGTAAACAAACAAGTATAAGAAATAAAGC
ATACAAATTAAAAGGAAAGGGTTTTGGTATACAGAAGTCTTATTACCAAAATGTGGGCTG
ATTTTGGCGAAAGTTGTTTTCCTTCTTCACAAACTACAAAGTCAGCGACCAACTCCACAG
TACCTACATATTAAAATAAGAAGAAGAAATAAGTTTTTGGATTAATATAGATAGCAAAAG
TGAGTATGAGAAAGAGGAATATTAAGATGGTGAGACGACAGACCTTTGTTTAGTCGTACA
GGCATTTCAAGCTTCCGAAGAAGTGGTTCCATCTCATGTGTGAACTGTTCTAGAGGACCT
TCCTTCAGTTCAACCTAATCATTTAGGTGGTACCAATATAGTAATTAAGATAAAAGTAAC
GAAACAAAGTTTTTTAAATAACAAAGAGAAGCAGAGCCGNATCATA

Last Updated on 06.12.2018 16:11