Sequences from confirmation amplicon


Confirmation sequences for line 484G10 and hit At1g25520 are shown.
Line ID 484G10
Locus At1g25520 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:8962769
Primers used for PCR RK77 - 8474, show primer details
   
Sequence Name 35-K021337-022-484-G10C-8409
GenBank Accession LN501656 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>35-K021337-022-484-G10C-8409
TTTCCCGGACATGAAGCCATTTACAACAGAAGTATCTTACACACCTTCAAAGAGCCTGTG
GCAGATTCTAAACTACCAAAACACTATGAAACTGAATGGCAATATAAAAGCTACAGACAA
AGGATCCAAAGATGCCATCTAGATTCTTAATCAGGAAAAGGCAATCCAATAAGTAAGACT
TTAGACTTCGATTCATGTAAAAGCCATTGACTTCATCGACCTTAAACCCTCAATAGATTA
CATGAAGCAACAAAATCTAAAACTAAAGCGAAGAGGAGACGAAACACTTACTGCTGCAGC
GAAAAACGTCTTGTCACCAATCTCCGATACAAAGGTCATAGCAAGTGACTTCGTAAAACC
CTATAAAACCCCAACAATCATAAACCCAGATTAGCTCCACAACGATTCTTACGAGTTTAC
TCCCCAGAAAACATTAATCTTTTACTCTACCTGCAAAACCGAGCTCATGGCTGCCGAATT
ATAAAATCGACGGCGAATTAGCTACGAAGGAGAAGAAAAAAACGAAACTTTCGATTTTTC
TCCGGCTGTGTTACAAAGAAGACGGAGTGATCAATCGCCGGAGTTAATTAACAGTCGTCG
AGAGAGAGCAAATAGACGAGACGAACATTCGCT
   
Sequence Name 36-K021337-022-484-G10C-RK77
GenBank Accession LN502054 [GenBank]
Sequencing primer gene specific primer, RK77: 5'-ATCTTTTAGCTCTTCAGCGAATGT-3'
Sequence (A. th genome BLAST matches underlined) 
>36-K021337-022-484-G10C-RK77
CGTCTATTTGCTCTCTCTCGACGACTGTTAATTAACTCCGGCGATTGATCACTCCGTCTT
CTTTGTAACACAGCCGGAGAAAAATCGAAAGTTTCGTTTTTTTCTTCTCCTTCGTAGCTA
ATTCGCCGTCGATTTTATAATTCGGCAGCCATGAGCTCGGTTTTGCAGGTAGAGTAAAAG
ATTAATGTTTTCTGGGGAGTAAACTCGTAAGAATCGTTGTGGAGCTAATCTGGGTTTATG
ATTGTTGGGGTTTTATAGGGTTTTACGAAGTCACTTGCTATGACCTTTGTATCGGAGATT
GGTGACAAGACGTTTTTCGCTGCAGCAGTAAGTGTTTCGTCTCCTCTTCGCTTTAGTTTT
AGATTTTGTTGCTTCATGTAATCTATTGAGGGTTTAAGGTCGATGAAGTCAATGGCTTTT
ACATGAATCGAAGTCTAAAGTCTTACTTATTGGATTGCCTTT

Last Updated on 06.12.2018 16:11