Sequences from confirmation amplicon


Confirmation sequences for line 192A09 and hit At1g29690 are shown.
Line ID 192A09
Locus At1g29690 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:10380574
Primers used for PCR JY01 - 8474, show primer details
   
Sequence Name 33-K023373-022-192-A09C-8409
GenBank Accession LN500828 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>33-K023373-022-192-A09C-8409
GTAGATTTCCCGGACATGATAAAACCGGTAATAGATTGACTCTCTGCTTCATGAAATCTG
TGCTTTATCATGTCATTCACATAGTTTTCTATTTCAGATACTGGTAAAGGAGAAGACTGA
TGTTGTCTGATGTATACTACATCTCTTCCACCAATGGTCACCGAAGTTACAATATGAGTG
CCGTAATTTTCAATAAAGCTGTACCAAAAGAAGGAACAAGTGGAGAAACCACATAAGAAT
CATGATAGATGTCATCAAGAATCACATAAAAGTGTAGCAAAAGGACAACAAAAGGAAACT
GACTCTCAATTAGAGTGTGAACCTTTGAAGCCATGTTCATGTAAACAGCGAGAACAGAAC
ATACAGAAAAGCAGAAAACATGGTGTATTAAAGAGAGGGAATATACCTAGCTAGGGAAGC
AGGATCCCACGAGGAAGGAACAGCACGTCTGATCTCATTGTGCAAAACCAATGTAAGCTT
TGCTAATTTGACATCATAAAGCGGGATAAAATATCCAACCAGTG
   
Sequence Name 34-K023373-022-192-A09C-JY01
GenBank Accession LN501252 [GenBank]
Sequencing primer gene specific primer, JY01: 5'-TAAATCTCTTGCACTGGTTGGATA-3'
Sequence (A. th genome BLAST matches underlined) 
>34-K023373-022-192-A09C-JY01
TTTTATCCCGCCTTTATGATGTCAAATTAGCAAAGCTTACATTGGTTTTGCACAATGAGA
TCAGACGTGCTGTTCCTTCCTCGTGGGATCCTGCTTCCCTAGCTAGGTATATTCCCTCTC
TTTAATACACCATGTTTTCTGCTTTTCTGTATGTTCTGTTCTCGCTGTTTACATGAACAT
GGCTTCAAAGGTTCACACTCTAATTGAGAGTCAGTTTCCTTTTGTTGTCCTTTTGCTACA
CTTTTATGTGATTCTTGATGACATCTATCATGATTCTTATGTGGTTTCTCCACTTGTTCC
TTCTTTTGGTACAGCTTTATTGAAAATTACGGCACTCATATTGTAACTTCGGTGACCATT
GGTGGAAGAGATGTAGTATACATCAGACAACATCAGTCTTCTCCTTTACCAGTATCTGAA
ATAGAAAACTATGTGAATGACATGATAAAGCACAGATTTCATGAAGCAGAGAGTCAATCT
ATTACCGGTTTTATCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAA
TATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCG
CAGCGTTATTATAAAATGAAAGTACATTTTTGATAAAACGACAAATTACGATCCGTCGTA
TTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATNTCGACGTGTC
T

Last Updated on 06.12.2018 16:11