Sequences from confirmation amplicon


Confirmation sequences for line 385H08 and hit At1g29690 are shown.
Line ID 385H08
Locus At1g29690 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:10379514
Primers used for PCR JY02 - 8474, show primer details
   
Sequence Name 45-K023495-022-385-H08C-8409
GenBank Accession LN505389 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>45-K023495-022-385-H08C-8409
GTAGATTTCCCGGACATGAAGCCATTTACAATTGAAACTGGAACCGGGAGGTCCATCTAC
ATCAAGTTCGACCGAGGAAGTGTCAGGGCAATCAGGAAAACTGGCCAAGATTGTGGATTC
GTCAGAGATGTTAAAAGGACCACAGGATTTACCTGGACATTGGCTTGTCACTGGAGCAAA
GCTAGGCGTTGAAAAGGGTAAGATTGTATTGCGTGTGAAGTATTCGTTGCTAAATTATTG
AGAGATTTTAGATGAGTTCTTGTTTTTCCGGCGGCCGGGAAAGTTTTCCGTCAGCATTCT
TTGCACAGCGAATCATTCTT
   
Sequence Name 46-K023495-022-385-H08C-JY02
GenBank Accession LN505736 [GenBank]
Sequencing primer gene specific primer, JY02: 5'-GAAACATGTACAAGAATGATTCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>46-K023495-022-385-H08C-JY02
CAAAGAATGCTGACGGAAAACTTTCCCGGCCGCCNGGAAAAACAAGAACTCATCTAAAAT
CTCTCAATAATTTAGCAACGAATACTTCACACGCAATACAATCTTACCCTTTTCAACGCC
TAGCTTTGCTCCAGTGACAAGCCAATGTCCAGGTAAATCCTGTGGTCCTTTTAACATCTC
TGACGAATCCACAATCTTGGCCAGTTTTCCTGATTGCCCTGACACTTCCTCGGTCGAACT
TGATGTAGATGGACCTCCCGGTTCCAGTTTCAATTGTAAATGGCTTCATGTCCGGGAAAT
CTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCA
ATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATT
TTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTAT
TCTAATTCGGAAATCTTTATTTCGACGTGTCTACATTCA

Last Updated on 06.12.2018 16:11