Sequences from confirmation amplicon


Confirmation sequences for line 552A03 and hit At1g29730 are shown.
Line ID 552A03
Locus At1g29730 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:10401417
Primers used for PCR DR32 - 8474, show primer details
   
Sequence Name 83-K035537-022-552-A03C-DR32
GenBank Accession LN514267 [GenBank]
Sequencing primer gene specific primer, DR32: 5'-TGGAGAACAATTGTCTTTCTGATG-3'
Sequence (A. th genome BLAST matches underlined) 
>83-K035537-022-552-A03C-DR32
CTGAAGATGATTGGACCCATCCTTTGACTGCATTCTCTTCTGTCTTGAGATTGAATATTT
GCTTGTAAACTGTGGGAGCAGGAAGAAGCTGTCTCAAACTAGAATGGGGAACAAGACACA
AAATATGCTTGGGAATCGCGAGAGGGCTTGCTTTCCTCCATGAAGATTCAGCAGTTAAGA
TCATTCACCGAGACATTAAAGGGACAAATGTTCTACTTGACAAGGATCTGAACTCAAAGA
TATCAGATTTCGGGTTGAACTCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAA
ATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTAC
CAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACA
TTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATT
ATTCTAATTCGGAAATCTTTATTTCGACGTGTCTACATTCACGTCCAAATGGG
   
Sequence Name 35-K035537-022-552-A03C-8409
GenBank Accession LN501737 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>35-K035537-022-552-A03C-8409
TTTAAATTGAATATATCCTGAGTTCAACCCGAAATCTGATATCTTTGAGTTCAGATCCTT
GTCAAGTAGAACATTTGTCCCTTTAATGTCTCGGTGAATGATCTTAACTGCTGAATCTTC
ATGGAGGAAAGCAAGCCCTCTCGCGATTCCCAAGCATATTTTGTGTCTTGTTCCCCATTC
TAGTTTGAGACAGCTTCTTCCTGCTCCCACAGTTTACAAGCAAATATTCAATCTCAAGAC
AGAAGAGAATGCAGTCAAAGGATTTGGTCCAATCATCTATCAGATAAATTTACCAAATAA
TGCATCAGAAAGACAATTGTTCT

Last Updated on 06.12.2018 16:11