Sequences from confirmation amplicon


Confirmation sequences for line 570G10 and hit At1g30400 are shown.
Line ID 570G10
Locus At1g30400 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:10733651
Primers used for PCR 26fp - 8474, show primer details
   
Sequence Name 63-K111394-0022-570-G10C-26fp
GenBank Accession LN511015 [GenBank]
Sequencing primer gene specific primer, 26fp: 5'-AGAAATGGATACTTCTCCTGGGAT-3'
Sequence (A. th genome BLAST matches underlined) 
>63-K111394-0022-570-G10C-26fp
TCTACTTCTATAGTTTCTATCTTACATATTGTCAATGAAGTACAAAAAGTGAGCTAGAAG
TATACATATGCAGGCGGATAGGCCAACATTGTCAAACATCAACCTGGACATACCTCTTGG
CAGCCTAGTTGCGGTAGTTGGCAGCACAGGAGAAGGAAAAACCTCCCTGATATCTGCTAT
GCTTGGGGAACTTCCTGCAAGATCTGATGCGACTGTTACTCTTAGAGGATCAGTCGCTTA
TGTTCCACAAGTTTCATGGATCTTTAACGCAACAGTAAGTTTATATATGCTACTCAGTTT
ATAGTATGGTTCTCAATGCGAAAATGTCAAATTCTCCTCTTGGATTGTTACTTATTTTGT
ATGTATTTTATGTTTTGTATATGATGATGTGTGCTTTTAGATACGTCCACATGCTGATGG
TTGTAATTAACATCGCGTAGGTACGTGACAATATATTGTTTGGGGCTCCTTTTGACCAAG
AAAAATATGAAAGGGTGATTGATGTGACAGCACTCCAGCAATGAGTATGATGGTCAATAT
GGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCA
   
Sequence Name 47-K111393-0022-570-G10C-8409
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>47-K111393-0022-570-G10C-8409
TTTTTCTTGGGTCAAAGGGAGCCCCAANNACAATATATTGGTCACGNNTACCTACGNNCG
ATGGGTTAATTACAACNNNCATCAGCATGGGTGGAANCGTATCTANNAAAGCACACATNN
CATCATATANNCAAAACATANNAAATACATNNNCAAAATAAGTNNAACAATCCAAGAAAA
GGAGAATTNNTGACATTNNTTCGCATTGANNGAACCATANNCTATAAACTNNNAGTAGCA
NNTATATAAANNCTTACTGGTTGNNCGTTAAAGAAATCCATGAAACNTNNTGTGGANNAC
ATAAGCGAACTGGGATCCTCTAAGAAGTAANNCAGTCGCATCAGAAATCTTGGCAGGANN
AGTTCCCCANNAGCATAGCAGAAATATCAGGGGAGGGGTTTTTCNNCTTCTCCTGGGTGC
TNNGCCAACTNNACCGCANNACTAGGCTGGGCCAAGAAGGTANNTGTCCAGGGTNNTGAT
GTTTGNANNCAATGTTNNGGCCTANNTCCGCCTGGGCATA

Last Updated on 06.12.2018 16:11