Sequences from confirmation amplicon


Confirmation sequences for line SALK_068977c and hit At1g31070 are shown.
Line ID SALK_068977c
Locus At1g31070 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:11085493
Primers used for PCR 34mh - LBb3, show primer details
   
Sequence Name 31-K115696-SALK_068977C-34mh
GenBank Accession LN485152 [GenBank]
Sequencing primer gene specific primer, 34mh: 5'-GATATTGAGGTTCGTGATTTCGAT-3'
Sequence (A. th genome BLAST matches underlined) 
>31-K115696-SALK_068977C-34mh
GGATCTCAGATTCAGTTAAGCGTAATTAACTGATAACACTGTTATTTTATTTTCTGCTTA
CAGAATTTGGATCTTCCAAGAATAGATCGGATCATTAGATGTTCACTTCACTCTCAAGGT
TTTTTAATTCGTTACGCTAATCAAATTAGTTAACATGCATTGTACTATAATCTCGGTTTT
TCTCGGTTAATTAGGTTTACCGGTTGCGGCGATTGAACCGGTACCGGAAAATTGGGTTTC
GACAGTAGATGGTAGAACAATGGAAGATAGAGAGAAATGGTGGAAAATGGGATTAAAAAC
TATCTATGACTTAATAACACATTGCGGACGTTTTTAATGTACTGGGGTGGTTTTTCTTTT
CACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCGGGCCCTGAAAGAGTTGCAG
CAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCAAAAATCCTGTTTGATGGTGGTTCCAAA
TCGGACAAA
   
Sequence Name 20-K115695-SALK_068977C-R210
GenBank Accession LN484858 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>20-K115695-SALK_068977C-R210
TTTTTATCCATTTTCCCCATTTCTCTCTATCTTCCATTGTTCTACCATCTACTGTCGAAA
CCCAATTCTCCGGTACCGGTTCAATCGCCGCAACCGGTAAACCTAATTAACCGAGAAAAA
CCGAGATTATAGTACAATGCATGTTAACTAATTTGATTAGCGTAACGAATTAAAAAACCT
TGAGAGTGAAGTGAACATCTAATGATCCGATCTATTCTTGGAAGATCCAAATTCTGTAAG
CAGAAAATAAAATAACAGTGTTATCAGTTAATTACGCTTAAACTGAATCTGAGATCCAAA
TCTTAACGAAGAATCTGGAAAAAAAAAATCGAAATCACGAACCCTCAATATC

Last Updated on 06.12.2018 16:11