Sequences from confirmation amplicon


Confirmation sequences for line 546H05 and hit At1g31470 are shown.
Line ID 546H05
Locus At1g31470 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:11265097
Primers used for PCR 19cv - 8474, show primer details
   
Sequence Name 29-K117188-0022-546-H05C-19cv
GenBank Accession LN499456 [GenBank]
Sequencing primer gene specific primer, 19cv: 5'-GAGGGAAATAACCTAAAGCAATCC-3'
Sequence (A. th genome BLAST matches underlined) 
>29-K117188-0022-546-H05C-19cv
GGCGAGGTAATTTAACCTCACCTGAGAAATTCCGAGAACTGATTTGAGATGGGAAGAGTA
AGCTGAGAAATCGAAGTTGGTTCCCGTCGATGCTTGGATCCAGATCGCTGCCACAAGAAC
AGTCCATTTCCGCCATCGACCGAACATGGTCCTGAGAGGCGGAAGATGTTTCCGGTGAAA
CTTTGTTTCTCCGTCGTCGAAGGAGGCTCGGTTAGGTCGGAGTTTATCTGAAACATCTCT
GATACGAGGTCTCATATACAAAGGAAGATGATTGATGTTGACCGATGCTTGCAAAAAAGC
GTTTAGTTGTTCATCTACCGCTTTTGATCTTTGTTCAATAAAAGGGATTTTGGGTTTTTG
ATGTTAATTTTTTTATTTTATTTTAACAAGTGTCGTATTTTAAATGTTCAATTGTAAATG
GCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAA
AAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCACGATTATT
ATAAAAA
   
Sequence Name 14-K117187-0022-546-H05C-8409
GenBank Accession LN492687 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>14-K117187-0022-546-H05C-8409
CATTTACATTGAACATTAAAATACGACACTTGTTAAAATAAAATAAAAAAATTAACATCA
AAAACCCAAAATCCCTTTTATTGAACAAAGATCAAAAGCGGTAGATGAACAACTAAACGC
TTTTTTGCAAGCATCGGTCAACATCAATCATCTTCCTTTGTATATGAGACCTCGTATCAG
AGATGTTTCAGATAAACTCCGACCTAACCGAGCCTCCTTCGACGACGGAGAAACAAAGTT
TCACCGGAAACATCTTCCGCCTCTCAGGACCATGTTCGGTCGATGGCGGAAATGGACTGT
TCTTGTGGCAGCGATCTGGATCCAAGCATCGACGGGAACCAACTTCGATTTCTCAGCTTA
CTCTTCCCATCTCAAATCAGTTCTCGGAATTTCTCAGGTGAGGTTAAATTACCTCGCCGT
GGCTTCTGATTTGGGGAAAGCGTTTGGATGGTCGTCTGGGATTGCTTTAGGTATTTTCCC
TC

Last Updated on 06.12.2018 16:11