Sequences from confirmation amplicon


Confirmation sequences for line 229D11 and hit At1g32230 are shown.
Line ID 229D11
Locus At1g32230 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:11613663
Primers used for PCR JK20 - 8474, show primer details
   
Sequence Name 03-K023017-022-229-D11C-8409
GenBank Accession LN487304 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>03-K023017-022-229-D11C-8409
GTAGATTTCCCGGACATGAAGCCATTTACAATTGAATATATCCTGACCAGCGCCTTCCCA
GAGCGATTCTCGTAAGCGCTAAGCTTGTTTTCACCTTCCAGCCTTCTTCTTTTGTCAGGA
ATTTGGCACTGCCCGTTCGGTGGGGGCACATTTTGCAGCTTTGCACACGAAACTCCAGTA
ACATATGCAGCATAGCTTGCTGCGCGCTTCCTCTTTTTTCCGAATCCATCTTCACACCTA
CTACTATCCAACACCTTGACGATCTTGGCTTCCATTTCTATATATTAACAATACTAAACC
TATAACCTTGATAGAAAAAGTCAACTGTGTAACCTCTAAACTCCCTAGCAGTCCTAAAGA
TAGTGTCTTGCCGACAGCATTAGAAAACACTGCACAAAAGAAATGGA
   
Sequence Name 04-K023017-022-229-D11C-JK20
GenBank Accession LN487745 [GenBank]
Sequencing primer gene specific primer, JK20: 5'-TTTAAGCCTTTTCCATTTCTTTTG-3'
Sequence (A. th genome BLAST matches underlined) 
>04-K023017-022-229-D11C-JK20
CAGTGTTTTCTAATGCTGTCGGCAAGACACTATCTTTAGGACTGCTAGGGAGTTTAGAGG
TTACACAGTTGACTTTTTCTATCAAGGTTATAGGTTTAGTATTGTTAATATATAGAAATG
GAAGCCAAGATCGTCAAGGTGTTGGATAGTAGTAGGTGTGAAGATGGATTCGGAAAAAAG
AGGAAGCGCGCAGCAAGCTATGCTGCATATGTTACTGGAGTTTCGTGTGCAAAGCTGCAA
AATGTGCCCCCACCGAACGGGCAGTGCCAAATTCCTGACAAAAGAAGAAGGCTGGAAGGT
GAAAACAAGCTTAGCGCTTACGAGAATCGCTCTGGGAAGGCGCTGGTCAGGATATATTCA
ATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAA
TATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCG
CAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTAT
TTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATTTCGACGTGTCT
ACAT

Last Updated on 06.12.2018 16:11