Sequences from confirmation amplicon


Confirmation sequences for line 347B03 and hit At1g32450 are shown.
Line ID 347B03
Locus At1g32450 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:11716202
Primers used for PCR CN13 - 8474, show primer details
   
Sequence Name 32-K030756-022-347-B03C-8409
GenBank Accession LN500460 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>32-K030756-022-347-B03C-8409
TCTACTGTAGATTTACTCATTGCTGCACTATAATCTACTCAGTCCTCTTCACTCAAATGG
CCTCTCTCTTTGTGGAGCAAGGCGCTGCAATGAATACCTCTGTCTCAGATTTCAAAATCC
CCCCGGCAAGTATGTCCAGCTTCGACATCCTTAGCGTCGCTTTGTTCATATTCCTGTACC
GCAGAGTTCTCGAGCCAGTAGCCAACAGATTCAAAAAGAATGGATCAAAAGGAATCACCG
AGCTTCACAGGATGGGAATCGGACTTGTGATTGCTGTCATAGCTATGATTGCAGCCGGGA
TTGTGGAATGCTATAGGCTTAAGTATGCAGACAAGAGTTGTACTCACTGTGATGGCTCAA
GCTCCTTAAGCATCTTCTGGCAGGTAATGAACACAGACCTTAAAACTCACTTTGGTCCTA
AAATCAACAACTTACAAAAGAAACTTCCGAGTATTGACACTCTTCTACTCTTTGCAGGCT
CCACAATACTCACTCATCGGGGCATCAGAAGTGTTCATGTACGTGGGTCAGCTTGAGTTC
TTCAACGCGCAAACACCAGACGGACTAAAGAGCTTCGGGAGCGCTCTGTGTATGATGTCA
ATGTCAATGGGGAACTTTTGTTA
   
Sequence Name 71-K030756-022-347-B03C-CN13
GenBank Accession LN512485 [GenBank]
Sequencing primer gene specific primer, CN13: 5'-CTAACAAAGTTCCCCATTGACATT-3'
Sequence (A. th genome BLAST matches underlined) 
>71-K030756-022-347-B03C-CN13
GAGCTCTTTAGTCGTCTGGTGTTTGCGCGCTTGAAGAACTCAAGCTGACCCACGTACATG
AACACTTCTGATGCCCCGATGAGTGAGTATTGTGGAGCCTGCAAAGAGTAGAAGAGTGTC
AATACTCGGAAGTTTCTTTTGTAAGTTGTTGATTTTAGGACCAAAGTGAGTTTTAAGGTC
TGTGTTCATTACCTGCCAGAAGATGCTTAAGGAGCTTGAGCCATCACAGTGAGTACAACT
CTTGTCTGCATACTTAAGCCTATAGCATTCCACAATCCCGGCTGCAATCATAGCTATGAC
AGCAATCACAAGTCCGATTCCCATCCTGTGAAGCTCGGTGATTCCTTTTGATCCATTCTT
TTTGAATCTGTTGGCTACTGGCTCGAGAACTCTGCGGTACAGGAATATGAACAAAGCGAC
GCTAAGGATGTCGAAGCTGGACATACTTGCCGGGGGGATTTTGAAATCTGAGACAGAGGT
ATTCATTGCAGCGCCTTGCTCCACAAAGAGAGAGGCCATTTGAGTGAAGACGACTGAGTA
GATTATAGTGCAGCAATGAGTAAATCTACATGTAGATGTTTCATTTTCCGGGAAATCTAC
ATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTT
TTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGA
TAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTA
ATTCGGAAATCTTTATTTCGACGTGTCTAC

Last Updated on 06.12.2018 16:11