Sequences from confirmation amplicon


Confirmation sequences for line 674B07 and hit At1g36160 are shown.
Line ID 674B07
Locus At1g36160 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:13538775
Primers used for PCR GE38 - 8474, show primer details
   
Sequence Name 63-K100940-0022-674-B07C-8409
GenBank Accession LN510939 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>63-K100940-0022-674-B07C-8409
CATTTACATTGAATATATCCTCTCTCTTTCTCATCACAAGAAGATAAATGTGCCTGTTGC
AAGAAGTAGACAGAAAAAATTCAGAGAGTAAGAAACAGGATGGTAATTCTAATGGAAGAA
ATAATAGTCAATTACCTCAAGAATGCCTTTTAAAAGTTTGGCAGGGAAATCGGTGGTCAA
AGAGTTTCTGGAAATACTCTCAAATTCCCTATACTTTGATTCTAGCTGATAAAGAGTTTT
ATTTAGATATGATTAAAAGTCTTGAACTCAAAACAAATATCAATATATAAGAAACCGGAT
TATGAATACTACACAGGTGTTTACCATGTTCCTGAGATTTTTAGGTAGTCGTGTCGCCAG
AACTGCAAAGCACTCTTGCCACTGAAGAAATGGGAGTTCAGGGCTATCAAGGCAATTAAG
TAAGTCTTGAACAACCTGCAACGTATGAGCTATCTCATAGTCAAATAATCTAGTAGAAAA
GTTGGATCAAATAGGAAAAACAAACAGTGTTTACCTCATCTACTTTAT
   
Sequence Name 63-K100947-0022-674-B07C-GE38
GenBank Accession LN510940 [GenBank]
Sequencing primer gene specific primer, GE38: 5'-TGCTGGCTATGAGCATAAAGTAGA-3'
Sequence (A. th genome BLAST matches underlined) 
>63-K100947-0022-674-B07C-GE38
CTATTTGATCCACTTTTCTACTAGATTATTTGACTATGAGATAGCTCATACGTTGCAGGT
TGTTCAAGACTTACTTAATTGCCTTGATAGCCCTGAACTCCCATTTCTTCAGTGGCAAGA
GTGCTTTGCAGTTCTGGCGACACGACTACCTAAAAATCTCAGGAACATGGTAAACACCTG
TGTAGTATTCATAATCCGGTTTCTTATATATTGATATTTGTTTTGAGTTCAAGACTTTTA
ATCATATCTAAATAAAACTCTTTATCAGCTAGAATCAAAGTATAGGGAATTTGAGAGTAT
TTCCAGAAACTCTTTGACCACCGATTTCCCTGCCAAACTTTTAAAAGGCATTCTTGAGGT
AATTGACTATTATTTCTTCCATTAGAATTACCATCCTGTTTCTTACTCTCTGAATTTTTT
CTGTCTACTTCTTGCAACAGGCACATTTATCTTCTTGTGATGAGAAAGAGAGAGGATATA
TTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGG
TCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATT
C
Primers used for PCR GE39 - 8474, show primer details
   
Sequence Name 72-K100941-0022-674-B07C-8409
GenBank Accession LN512686 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>72-K100941-0022-674-B07C-8409
ATTTACAATTGATCATTGCTTGCCCTTGAAAGGCTCATTGAACCATTGATGAGCCTTGCA
AAATCTTATGAAGGTGGTAGAGAAAGTCATGCCCGTGTTATTGTTCATTCTCTCTTTGAA
GAATATCTATCAGTAGAAGAATTATTCAATGATAACATGCTGGTATTATATGGCTCAATA
GCTAATTAACAGATTTTTGGTTACTAGCGATGTCTGAGGCGTCTAAATAATCATTTTATT
TTTCTTGAATAGGCTGATGTTATAGAACGCATGCGTCAGCTATACAAGAAAGATCTGTTG
AAAATTGTGGATATAGTGCTCTCACACCAGGTCTGTGATCATCTTTCTCAGA
   
Sequence Name 72-K100949-0022-674-B07C-GE39
GenBank Accession LN512687 [GenBank]
Sequencing primer gene specific primer, GE39: 5'-GAAAAACCTGGTCTGAGAAAGATG-3'
Sequence (A. th genome BLAST matches underlined) 
>72-K100949-0022-674-B07C-GE39
ATATCCCAATTTTCACAGATCTTTCTTGTATAGCTGACGCATGCGTTCTATAACATCAGC
CTATTCAAGAAAAATAAAATGATTATTTAGACGCCTCAGACATCGCTAGTAACCAAAAAT
CTGTTAATTAGCTATTGAGCCATATAATACCAGCATGTTATCATTGAATAATTCTTCTAC
TGATAGATATTCTTCAAAGAGAGAATGAACAATAACACGGGCATGACTTTCTCTACCACC
TTCATAAGATTTTGCAAGGCTCATCAATGGTTCAATGAGCCTTTCAAGGGCAAGCAATGA
TCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGT
CAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGT
C

Last Updated on 06.12.2018 16:11