Sequences from confirmation amplicon


Confirmation sequences for line 048C12 and hit At1g41820 are shown.
Line ID 048C12
Locus At1g41820 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:15583645
Primers used for PCR CW55 - 8474, show primer details
   
Sequence Name 68-K104127-0022-048-C12C-CW55
GenBank Accession LN512004 [GenBank]
Sequencing primer gene specific primer, CW55: 5'-AATAATTGATGCCAAACATAAGCC-3'
Sequence (A. th genome BLAST matches underlined) 
>68-K104127-0022-048-C12C-CW55
AGGAATAGAGCAATATGAGCAGATCTCGCATCAAAAAGAGGCAAAACCAAATAGACCCCA
AGAGAAGCATAATTGGAGGAGTAGACCAACCAAAGATGAATCAAATTGTTACTACTATAT
TCGTGATGATCATAGACGTTTTGAGCAAGCTAGACAACAACCTAATGATGTGAAAGAAGT
TAAGACCATGAAGCAAAACCGAAAGGAAAGTCTATCCTCAAATGAACAGTTAAAGGATGA
GCTTCTTATGATTCTCAACGCTTACAACAAACTGAAGAAAGCCAAGCATCCTCTTCCTTC
TAATTCAAAAAAAGCTAAGATTGCTCTTCCTTCCGAATTTACCAAAGATACTTGTAAATG
GCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAA
AAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATATAGATGTCCGCACGGTTTAT
A
   
Sequence Name 81-K104128-0022-048-C12C-8409
GenBank Accession LN514062 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>81-K104128-0022-048-C12C-8409
TGAGCCATTTACAAGTATCTTTGGTAAATTCGGAAGGAAGAGCAATCTTAGCTTTTTTTG
AATTAGAAGGAAGAGGATGCTTGGCTTTCTTCAGTTTGTTGTAAGCGTTGAGAATCATAA
GAAGCTCATCCTTTAACTGTTCATTTGAGGATAGACTTTCCTTTCGGTTTTGCTTCATGG
TCTTAACTTCTTTCACATCATTAGGTTGTTGTCTAGCTTGCTCAAAACGTCTATGATCAT
CACGAATATAGTAGTAACAATTTGATTCATCTTTGGTTGGTCTACTCCTCCAATTATGCT
TCTCTTGGGGTCTATTTGGTTTTGCCTCTTTTTGATGCGAGATCTGCTCATATTGCTCTA
TTCCTTTGAGTTGGTCGTTGATCGGCTTATGTTTGGCTCCAATTA

Last Updated on 06.12.2018 16:11