Sequences from confirmation amplicon


Confirmation sequences for line 399E08 and hit At1g43020 are shown.
Line ID 399E08
Locus At1g43020 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:16156883
Primers used for PCR 22zf - 8474, show primer details
   
Sequence Name 21-K125972-0022-399-E08C-22zf
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer gene specific primer, 22zf: 5'-TTAATTCAAACGCACTCAAGACG-3'
Sequence (A. th genome BLAST matches underlined) 
>21-K125972-0022-399-E08C-22zf
ATATTTTACCATAAAGTACACGTATATAATGCTAACAAAATAACTGTTTATATTATATCT
TCTCTTTGTACTGTTACTTTTGTGTGTGTTTTTTATTGACGTCTTGACTCGAATTCTTAT
TTGGTATGGTGTTATTTTAACAACTTGGATTGATCTGCGAGTTAGGTTATAGACATGGAC
ATACATTGTATTTTGTTCTTAATATTCTATATTACAATCGCAAACTTTATGCTTCAGCAA
TATCTTGGCACAAAAACAAAAACAATATTTAGACGAACCATAGAGTGACTAGGTACAAAA
GATGGTTTTCAAATTTCAAGACTGCCTCTCACAAGTTCCGAATGGATGACATAACGGAAC
TTGGATTCATCGTGAACCCAACGAATGCATCTCTCTTGCTTTTTGTTCACAATTCTTCTC
ATTGCCGTTCTTTGTGACTCCATTAAACACTCAGACACGGTACTAAAGAGTTCACCCATG
TCAAGAGAAGTATCCTTCGCATAGTTGTAAATGATCTTTGGCAATAAAATCTTTGTCTCT
TTCTCAAATCCAACACTTGTTTGGATGTAACTGTCTCTTGCTTGCCTAAGTTCTTGAAAG
ATTCCTTCTGATGTATACACTCTGACCTAATCAAAACAATGTATATGGTTAATTAACATG
ATTTGTGAAGAGATTTAAAGGAGAAAGGAGTTGAGTCCGTATAGTACCATTGGATCGGTT
GATGCTCCGGTCGAGAGGGCAAAATGAAGAAGTGGCTCGGCATAGTCCAACGCGTAAGTG
TGTCTACCACTACTTGTCTTTGAACTCTTAGCCGGTGAAAACAGCGTCCGTAAACGCTAT
CATCGGCAAATAGAAATATTTCAAGATTGAGAATTCATAGACATGAATTGAATCCACAAT
ATTTTCAAAATGTTTTCAATTTTA
   
Sequence Name 07-K125971-0022-399-E08C-8409
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>07-K125971-0022-399-E08C-8409
AATTGAATATATCCTGGAAAAGAAAAGAAAAAGAAAAAGAAATGAATCAGCAATTGAAAT
GATAGTTTGTTGTTAGTTCATGTTAGTTCGTTGATTTGACCTCATATAACTTATGTGTTT
ATCCTCTCCAGGCAGCATTTAACATAGGTGGGGAGTGGGTAAATGCATACGATATCCAGA
GTTCGATATTGGGCATTCGTCCATGCCATTCACCATCAGTAAGTTGATTTATGACTTAAC
TTGTTTGCATACGTTTGTATGTGTTTCTCCTTTTGCAAAAGAATATGTAATAATTTGTCA
AATGGCGAAAAAGTAAATGAAAATTTTTAGAAAAGTATCCAAATTTTCTACTTCCTAAAA
TTGAAAACATTTTGAAAATATTGTGGATTCAATTCATGTCTATGAATTCTCAATCTTGAA
ATATTTCTATTTGCCGATGATAGCGTTTACGGACGCTGTTTTCACCGGCTAAGAGTTCAA
AGACAAGTAGTGGTAGACACACTTACGCGTTGGACTATGCCGAGCCACTTCTTCATTTTG
CCCTCTCGACCGGAGCATCAACCGATCCAATGGTACTATACGGACTCAACTCCTTTCTCC
TTTAAATCTCTTCACAAATCATGTTAATTAACCATATACATTGTTTTGATTAGGTCAGAG
TGTATACATCAGAAGGAATCTTTCAAGAACTTAGGCAAGCAAGAGACAGTTACATCCAAA
CAAGTGTTGGATTTGAGAAAGAGACAAAGATTTTATTGCCAAAGATCATTTACAACTATG
CGAAGGATACTTCTCTTGACATGGGTGAACTCTTTAGTACCGTGTCTGAGTGTTTAATGG
AGTCACAAAGAACGGCAATGAGAAGAATTGTGAACAAAAAGCAAGAGAGATGCATTCGTT
GGGTTCACGATGAATCCAAGTTCCGTTATG

Last Updated on 06.12.2018 16:11