Sequences from confirmation amplicon


Confirmation sequences for line 518E09 and hit At1g48590 are shown.
Line ID 518E09
Locus At1g48590 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:17964232
Primers used for PCR 68ys - 8474, show primer details
   
Sequence Name 50-K122663-0022-518-E09C-68ys
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer gene specific primer, 68ys: 5'-ACCGTAATCTATCTTTACGCATCG-3'
Sequence (A. th genome BLAST matches underlined) 
>50-K122663-0022-518-E09C-68ys
ATTTCGAGAATCATTATTTAACGCAAAGATAGTAGTTATGATAAACGAATGAGAAAATTG
AATGTGCAGAAATTGAAGACTCGAGTTATTTACAAAAATGTAAATCCTGAGTGGAATGAA
GACTTGACTCTCTCAGTTTCAGACCCCAATCTTACCGTCCTCTTGGTAAGGAACTACCAA
TTCTACGATCATATTTTTCATTTTTTATTTTCAAATCGCCATAATGTTACGTAAAAAAAA
AACATGATTACGGTGTCAATTTGCCTAGACGGTGTATGATTATGACACGTTCACCAAAGA
CGACAAGATGGGAGATGCAGAATTCGGGATTAAGCCGTTCGTTAATGCGTTGAAGATGCA
CTTACATGATCTCCCATCTGGGACCATCGTAACCACGGTCCAGCCTAGCCGCGACAACTG
CCTAGCCGAGGAGTCTCGGGTTATATGGTCTGATGGTAAGCTCGTTCAAGATATAGTTCT
TAGGCTGAGACATGTCGAATGTGGTGAGGTTGAGTATATATTCAATTGTAAATGGCTTCA
TGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAA
GAGTAATTACCAATTTTTTTTCAATTCAAAAATGT
   
Sequence Name 10-K122662-0022-518-E09C-8409
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>10-K122662-0022-518-E09C-8409
ATTTACATTGAATATATACTCAACCTCACCACATTCGACATGTCTCAGCCTAAGAACTAT
ATCTTGAACGAGCTTACCATCAGACCATATAACCCGAGACTCCTCGGCTAGGCAGTTGTC
GCGGCTAGGCTGGACCGTGGTTACGATGGTCCCAGATGGGAGATCATGTAAGTGCATCTT
CAACGCATTAACGAACGGCTTAATCCCGAATTCTGCATCTCCCATCTTGTCGTCTTTGGT
GAACGTGTCATAATCATACACCGTCTAGGCAAATTGACACCGTAATCATGTTTTTTTTTT
ACGTAACATTATGGCGATTTGAAAATAAAAAATGAAAAATATGATCGTAGAATTGGTAGT
TCCTTACCAAGAGGACGGTAAGATTGGGGTCTGAAACTGAGAGAGTCAAGTCTTCATTCC
ACTCAGGATTTACATTTTTGTAAATAACTCGAGTCTTCAATTTCTGCACATTCAATTTTC
TCATTCGTTTATCATAACTACTATCTTTGCGTTAAATAATGATTCTCGAAATATATAAAA
TACTAAGAATTTGTTGTTAGATAAATTCTACGATGCGTAAAGAAGATTTACG

Last Updated on 06.12.2018 16:11