Sequences from confirmation amplicon


Confirmation sequences for line 062C09 and hit At1g48920 are shown.
Line ID 062C09
Locus At1g48920 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:18100857
Primers used for PCR JY09 - 8474, show primer details
   
Sequence Name 40-K116478-0022-062-C09C-JY09
GenBank Accession LN503834 [GenBank]
Sequencing primer gene specific primer, JY09: 5'-CACCGAAGGTAGTCTTCTTACCTG-3'
Sequence (A. th genome BLAST matches underlined) 
>40-K116478-0022-062-C09C-JY09
ACATAAACCCAGAATTAGTGACATGTTAACTTGTTATAAAGAAGCTAGAAAACATGAATC
AAGAACAATTTATCTAACCTTGGGGAGTAAAACTAGGTCTTCCACGACCACGATCACTTC
CCCTGCCACCACCACTACCAAAACGACCACGACCTCCATCTCTTCCTCTGCCACCACCAC
TACCAAAACGACCACGACCTCCATCTCTTCCTCTGCCACCACCACTACCGAAACGACCAT
TGCCCCTTCCAAATCCACCACCACCACTGCTATCTCCTCTCGGTCTAGGCTCATCGACTA
CAAGATAGAATCCTCCTCCCATATCACTTCCATTTAGTTCCAACGCCTTCTCTTTGCCTT
CTGAAAACTCAAGGTAAGCAATTCTGAGGAGAAAAAAAACATTAATTTAGTTAGCACTTA
ACTTTCAATAGAGAAAATTCACAAACTAATGACAACTAAGAGAGAGTAATAATCACTAAC
CCTTTGGAGTTACCAGTGTCACGGTCAATTGGATATATTCAATTGTAAATGGCTTCATGT
CCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAG
TAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCACA
   
Sequence Name 15-K116477-0022-062-C09C-8409
GenBank Accession LN493137 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>15-K116477-0022-062-C09C-8409
CATTTACATTGAATATATCCAATTGACCGTGACACTGGTAACTCCAAAGGGTTAGTGATT
ATTACTCTCTCTTAGTTGTCATTAGTTTGTGAATTTTCTCTATTGAAAGTTAAGTGCTAA
CTAAATTAATGTTTTTTTTCTCCTCAGAATTGCTTACCTTGAGTTTTCAGAAGGCAAAGA
GAAGGCGTTGGAACTAAATGGAAGTGATATGGGAGGAGGATTCTATCTTGTAGTCGATGA
GCCTAGACCGAGAGGAGATAGCAGTGGTGGTGGTGGATTTGGAAGGGGCAATGGTCGTTT
CGGTAGTGGTGGTGGCAGAGGAAGAGATGGAGGTCGTGGTCGTTTTGGTAGTGGTGGTGG
CAGAGGAAGAGATGGAGGTCGTGGTCGTTTTGGTAGTGGTGGTGGCAGGGGAAGTGATCG
TGGTCGTGGAAGACCTAGTTTTACTCCCCAAGGTTAGATAAATTGTTCTTGATTCATGTT
TTCTAGCTTCTTTATAACAAGTTAACATGTCACTAATTCTTGGGTTTATGTTGGCTTTGC
AATATATGTTGCAGGTAAGAAGACTACCTTCGGTG

Last Updated on 06.12.2018 16:11