Sequences from confirmation amplicon


Confirmation sequences for line 255D04 and hit At1g50460 are shown.
Line ID 255D04
Locus At1g50460 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:18696420
Primers used for PCR KH77 - 8474, show primer details
   
Sequence Name 53-K118635-0022-255-D04C-KH77
GenBank Accession LN508307 [GenBank]
Sequencing primer gene specific primer, KH77: 5'-TTCAGTTTTCTCTATGAGCAGGTG-3'
Sequence (A. th genome BLAST matches underlined) 
>53-K118635-0022-255-D04C-KH77
GATCCGATACGGTTGTTGCGGTTGTATTTGGAACAGGTAGTAATGCATGTTACTTGGAAA
GAACCGATGCCATAATCAAGTGTCAGGGTCTGCTTACAACTTCTGGAAGCATGGTACTAA
GAATCATTGCATGGTCTATTTTTAGAAACCAGCTTTAGACCCAAAAGAAACATGTTTAAG
ATTGATTCCCTCTTATGTTTCAGGTGGTAAATATGGAGTGGGGAAATTGAAATGGTAATC
AATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCA
ATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCC
GCAGAGTTATTA
   
Sequence Name 13-K118634-0022-255-D04C-8409
GenBank Accession LN492264 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>13-K118634-0022-255-D04C-8409
CCATTTACAATTGATTACCATTTCAATTTCCCCACTCCATATTTACCACCTGAAACATAA
GAGGGAATCAATCTTAAACATGTTTCTTTTGGGTCTAAAGCTGGTTTCTAAAAATAGACC
ATGCAATGATTCTTAGTACCATGCTTCCAGAAGTTGTAAGCAGACCCTGACACTTGATTA
TGGCATCGGTTCTTTCCAAGTAACATGCATTACTACCTGTTCCAAATACAACCGCAACAA
CCGTATCTGGATCGTGATAATATCCAAGCGACAAGGCTCCAACAGTATCATTCACCTGCT
CATAAGAAAAACTGA

Last Updated on 06.12.2018 16:11