Sequences from confirmation amplicon


Confirmation sequences for line 028B07 and hit At1g52240 are shown.
Line ID 028B07
Locus At1g52240 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:19456941
Primers used for PCR BC12 - 8474, show primer details
   
Sequence Name 15-K104111-0022-028-B07C-BC12
GenBank Accession LN492947 [GenBank]
Sequencing primer gene specific primer, BC12: 5'-GTTCTCAACAACACACGAAAACAT-3'
Sequence (A. th genome BLAST matches underlined) 
>15-K104111-0022-028-B07C-BC12
ATTCTTGATGATGTTTTATGTGTTTATTAAATAACCTTAGGAAGTGACTCAAGGTAGCTT
TCAGGAATCTCCATTTCAGACAGAACTTGAGCGTTTATCGCCATTGCAGCTTTAAGGACT
TGATTCACACATTCCTTCTGGCTCTGTAGAAACCTTCTTGATATCTCTGAGAGGCCGTTA
GGTGGGACTTTTACTGCCGGTAACCACCATTTCTCATCGTTCCTTGTCTCGCAAGAATCA
GGAGAATCTTTTTTAACATAATAAAACTCATCCTGATCCTTGAATTTATCAAGACAATCC
TGCAAGAGAATGATTATAAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAG
CAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAAT
TCAAAAATGTAGATGTCCGCAGCGTTATT
   
Sequence Name 31-K104112-0022-028-B07C-8409
GenBank Accession LN500138 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>31-K104112-0022-028-B07C-8409
TTCGGACTGAGCCTTTACAATTTATAATCATTCTCTTGCAGGATTGTCTTGATAAATTCA
AGGATCAGGATGAGTTTTATTATGTTAAAAAAGATTCTCCTGATTCTTGCGAGACAAGGA
ACGATGAGAAATGGTGGTTACCGGCAGTAAAAGTCCCACCTAACGGCCTCTCAGAGATAT
CAAGAAGGTTTCTACAGAGCCAGAAGGAATGTGTGAATCAAGTCCTTAAAGCTGCAATGG
CGATAAACGCTCAAGTTCTGTCTGAAATGGAGATTCCTGAAAGCTACCTTGAGTCACTTC
CTAAGGTTATTTAATAAACACATAAAAACATTCATCAAGAATCTGTTATTGATCTAGTCC
TTATCGATGTTTTCGTGTGTTGTTGAGA

Last Updated on 06.12.2018 16:11