Sequences from confirmation amplicon


Confirmation sequences for line 960F01 and hit At1g52270 are shown.
Line ID 960F01
Locus At1g52270 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:19463778
Primers used for PCR 35tq - 8474, show primer details
   
Sequence Name 39-K116871w-0022-960-F01C-35tq
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer gene specific primer, 35tq: 5'-GATACACAATCGAATCAGAGCAAA-3'
Sequence (A. th genome BLAST matches underlined) 
>39-K116871w-0022-960-F01C-35tq
ATACAAGGGTTTACACAACGAACTTAAATAGAATCCAACAGACATGAAAGTCAAGAGTTG
TAACTAACTTACATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATAT
GGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCC
   
Sequence Name 19-K116870w-0022-960-F01C-8409
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>19-K116870w-0022-960-F01C-8409
ACTCTTGACTTTCATGTCTGTTGGATTCTATTTAAGTTCGTTGTTGTAAACCTTGTAATA
CAGTGTTTCCCATTCAATTAAAGTCAGTTTCTCTTTTGCTCTGATTCGATTGTGTAT
Primers used for PCR 15ui - 3144, show primer details
   
Sequence Name 28-K117104-0022-960-F01C-15ui
GenBank Accession LN499015 [GenBank]
Sequencing primer gene specific primer, 15ui: 5'-AATCATTCATCGTCATCCTTTTCT-3'
Sequence (A. th genome BLAST matches underlined) 
>28-K117104-0022-960-F01C-15ui
CACAATGACGACGCCCGTGAAGAAAGCCTCGCCATTAGACCGGTGGCGTTCTATAGAAAC
GGGCTTCCTCGTCCTCGTTTCTTCGATAATCATAGGTTCAACTCTTACCGTGTTGATACT
CCCCTTTCTGTGCTAGATCCACTTCTCTCATGGGCACAGCTGGAGAAATCTAGCCCTGCT
GCTCCGATTGTGCTGAAACGAAGAAGGTACTTAGATGATGAGGAAATCGGATCTGAAGAT
GTAGGTGTGGTTAGAATCAGGAGAAAACTCTCTGATGATTTTGATAGAGTCGCGGAAGAG
AGCAAGACCGAACTTGTTGAAGCTAACGAGAAGAAGAAGATCGAACAAGACAAGTTCAAC
GAGGTTATCTCCAAGATTGTTCATATTTAGTCGCTTCAATAAATTCTTGTTGGTCGTAAT
TGAGTTTCTAATGTTTGTAATGTAGTTAGTTATTAATCAAGTGTCGTGTCTATCAAACAC
TGATAGTTTAAACCGAAGGCGGGAAACGACAATCTGATCCCCGGGTACCCTGTCCTCTCC
AAATGAAATGAACTTCCTTATATAGAGGAAGGGTCTTGCGAAGGATAGTGGGATTGTGCG
TCATCCCTTACGTCAGTGGAGATAT
   
Sequence Name 12-K117103-0022-960-F01C-3144
GenBank Accession LN491760 [GenBank]
Sequencing primer T-DNA primer, 35St: 5'-GTGGATTGATGTGATATCTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>12-K117103-0022-960-F01C-3144
CGCAGACCCTTCCTCTATAAAGGAAGTTCATTTCATTTGGAGAGGACAGGGTACCCGGGG
ATCAGATTGTCGTTTCCCGCCTTCGGTTTAAACTATCAGTGTTTGATAGACACGACACTT
GATTAATAACTAACTACATTACAAACATTAGAAACTCAATTACGACCAACAAGAATTTAT
TGAAGCGACTAAATATGAACAATCTTGGAGATAACCTCGTTGAACTTGTCTTGTTCGATC
TTCTTCTTCTCGTTAGCTTCAACAAGTTCGGTCTTGCTCTCTTCCGCGACTCTATCAAAA
TCATCAGAGAGTTTTCTCCTGATTCTAACCACACCTACATCTTCAGATCCGATTTCCTCA
TCATCTAAGTACCTTCTTCGTTTCAGCACAATCGGAGCAGCAGGGCTAGATTTCTCCAGC
TGTGCCCATGAGAGAAGTGGATCTAGCACAGAAAGGGGAGTATCAACACGGTAAGAGTTG
AACCTATGATTATCGAAGAAACGAGGACGAGGAAGCCCGTTTCTATAGAACGCCACCGGT
CTAATGGCGAGGCTTTTCTTCACGGGCGTCGTCATTGTGTTTATTTCAGAAAGAGAGAGA
GAGACTGATAAAAG
Primers used for PCR 14ui - 8474, show primer details
   
Sequence Name 15-K117142-0022-960-F01C-14ui
GenBank Accession LN493149 [GenBank]
Sequencing primer gene specific primer, 14ui: 5'-TTTTGAACATATGTGTAAATCTAT-3'
Sequence (A. th genome BLAST matches underlined) 
>15-K117142-0022-960-F01C-14ui
AGTCATGAGTAAATTATTTTGATGCATACAGTTACTTTGTCAGAGTTGATGGATAAAGTC
ATATTTATAAGCACAAAAACAAACAAACCAAGTAGCATTGTTATGAACTATACTCATGAA
AATCTAAACCCTAGAGAGAATTGTTAGAAGCAAAGACTTCTTATTGTCTCAAAGAACAAA
ACAAGTGTTTACTTGTTGTTCTGTTTCTTATGAGATACACAATCGAATCAGAGCAAAAGA
GAAACTGACTTTAATTGAATGGGAAACACTGTATTACAAGGTTTACAACAACGAACTTAA
ATAGAATCCAACAGACATGAAAGTCAAGAGTTGTAACTAACTTACATGTCCGGGAAATCT
ACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAAT
TTTTTTTCAATTCAAAAATGTAGATGTCCGCACGGTTATTAT
   
Sequence Name 05-K117141-0022-960-F01C-8409
GenBank Accession LN488524 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>05-K117141-0022-960-F01C-8409
TACACTCTTGACTTTCTGTCTGTTGGATTCTATTTAAGTTCGTTGTTGTAAACCTTGTAA
TACAGTGTTTCCCATTCAATTAAAGTCAGTTTCTCTTTTGCTCTGATTCGATTGTGTATC
TCATAAGAAACAGAACAACAAGTAAACACTTGTTTTGTTCTTTGAGACAATAAGAAGTCT
TTGCTTCTAACAATTCTCTCTAGGGTTTAGATTTTCATGAGTATAGTTCATAACAATGCT
ACTTGGTTTGTTTGTTTTTGTGCTTATAAATATGACTTTATCCATCAACTCTGACAAAGT
AACTTGTATGCATCAAAATAATTTTACTCATTGACTTGATTAATCAAATTCATGACTTTC
ATAGATTTACACATATGTTCAAAAAGNNANGAGNGAGTCCTTACCATTTTTTTTCAATTC
ATAAATG

Last Updated on 06.12.2018 16:11