Sequences from confirmation amplicon


Confirmation sequences for line 866A01 and hit At1g53360 are shown.
Line ID 866A01
Locus At1g53360 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:19907361
Primers used for PCR 05ts - 8474, show primer details
   
Sequence Name 19-K117167-0022-866-A01C-05ts
GenBank Accession LN494933 [GenBank]
Sequencing primer gene specific primer, 05ts: 5'-AGACTTAGTCAGGGACTGCTCTGT-3'
Sequence (A. th genome BLAST matches underlined) 
>19-K117167-0022-866-A01C-05ts
GATCGCACGAACCTAGCTATAGACTTTGCCGGGACTCTAGAAAACATTTCAATAAGAAGA
TATACTAGGATTATGTCTGACTATTCTCTTACCGAGTTCGAAGATTATGTCATCAATAGA
TCTTCTGAGAAATGCTCCTCTAGATTTTTCAAGGCAGATATGTTTCAAACCCTAGAATCA
CCCTTTCAGAGCAAAGTCGACTTTGGTTGTTTATATTCAATTGTAAATGGCTTCATGTCC
GGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTA
ATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCG
   
Sequence Name 08-K117166-0022-866-A01C-8409
GenBank Accession LN489904 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>08-K117166-0022-866-A01C-8409
TTGAATATAAACACCAAAGTCGACTTTGCTCTGAAAGGGTGATTCTAGGGTTTGAAACAT
ATCTGCCTTGAAAAATCTAGAGGAGCATTTCTCAGAAGATCTATTGATGACATAATCTTC
GAACTCGGTAAGAGAATAGTCAGACATAATCCTAGTATATCTTCTTATTGAAATGTTTTC
TAGAGTCCCGGCAAAGTCTATAGCTAGGTTTCGTTGCGTATCGGAATCCATTCATCGTCG
TCCTGATGTCACAGAGCAGTCCCTGACTAAGTCT

Last Updated on 06.12.2018 16:11