Sequences from confirmation amplicon


Confirmation sequences for line 164A09 and hit At1g56570 are shown.
Line ID 164A09
Locus At1g56570 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:21196342
Primers used for PCR BH50 - 8474, show primer details
   
Sequence Name 19-K104153-0022-164-A09C-BH50
GenBank Accession LN494737 [GenBank]
Sequencing primer gene specific primer, BH50: 5'-ACTCAACGTTCTTGTTAAACCCTC-3'
Sequence (A. th genome BLAST matches underlined) 
>19-K104153-0022-164-A09C-BH50
TGCTGACACAGTTCATGCCGCTATATTGGCACAAGCAGCAACTAAACTCGTGAAAGTGTA
ACAATTTGGCACAAAGCCTTGCGATTCAAACCGCTGAAACATAAGCAGAGCTTCACTACT
ATCTGATCGCTCAAGCTCGGATATTAAAGTGTTCCATGTGATTAAATCTTTATCTTCCAT
CTCATGGAAATAGTGTTTCGCTTCTGATAAATAACCACACCTGCAATACAAGTCCAATAT
AGAGTTCATCACCGGGAGGTTAGACTGAAACCCACGTTTAATCACAGACGCATGAATTTG
CTTGCCGGTTGTAACTGAATCAATCGAAGCGGAAGCTCTAACCGCTATTGTTATACAGTA
AGGCGTCACTTCAGCATTCTCCTGAACACATAAAACAGATCCAAAATGTAAGAACTTCAA
TACAAAAACGTCACCAAAAACACGAGGAATATAGCTAAATTACCAGCAACATTTGCTTGT
ACATCTTTAAACCGCCNATGCCATCACCCAAATGCGTAAACCCGGTGATTAGAGTAGTCC
AAGCCATTTACCATCATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGAT
CAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTC
AATTCAAAAATGT
   
Sequence Name 19-K104154-0022-164-A09C-8409
GenBank Accession LN494738 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>19-K104154-0022-164-A09C-8409
GGAATGAGCCTTTACAATTGAATATGATGGTAAATGGCTTGGACTACTCTAATCACCGGG
TTTACGCATTTGGGTGATGGCATTGGCGGTTTAAAGATGTACAAGCAAATGTTGCTGGTA
ATTTAGCTATATTCCTCGTGTTTTTGGTGACGTTTTTGTATTGAAGTTCTTACATTTTGG
ATCTGTTTTATGTGTTCAGGAGAATGCTGAAGTGACGCCTTACTGTATAACAATAGCGGT
TAGAGCTTCCGCTTCGATTGATTCAGTTACAACCGGCAAGCAAATTCATGCGTCTGTGAT
TAAACGTGGGTTTCAGTCTAACCTCCCGGTGATGAACTCTATATTGGACTTGTATTGCAG
GTGTGGTTATTTATCAGAAGCGAAACACTATTTCCATGAGATGGAAGATAAAGATTTAAT
CACATGGAACACTTTAATATCCGAGCTTGAGCGATCAGATAGTAGTGAAGCTCTGCTTAT
GTTTCAGCGGTTTGAATCGCAAGGCTTTGTGCCAAATTGTTACACTTTCACGAGTTTAGT
TGCTGCTTGTGCCAATATAGCGGCATTGAACTGTGGTCAGCAACTTCATGGAAGGATTTT
TAGAAGAGGGTTTAACAA

Last Updated on 06.12.2018 16:11