Sequences from confirmation amplicon


Confirmation sequences for line 182C12 and hit At1g58250 are shown.
Line ID 182C12
Locus At1g58250 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:21598376
Primers used for PCR 32ra - 8474, show primer details
   
Sequence Name 83-K115895-0022-182-C12C-32ra
GenBank Accession LN514348 [GenBank]
Sequencing primer gene specific primer, 32ra: 5'-GCATAACATACTGTCCGCTCTACA-3'
Sequence (A. th genome BLAST matches underlined) 
>83-K115895-0022-182-C12C-32ra
TTTCTGGTGATAGGAAGAGATTGTCAAGTGTCTTAATTAGATCGATCAATATATTGGGAA
CTGAAAGATTCCATGATCTCCTAGGAAAATCGAAAAGTAAAACTACAAACAGAAATCCAA
GTATCAGAGCTTACTTATCTATATTGAAATCCACTCTGGATTATATATAAAACAAATCAC
ATATCTGAAGCTCAAAACTGAAAACACTTATCTACGTTATGAAGGATAGGGAAGTACCCC
AAATAGGTATGAAAAGAAACTATCAGGAATAAAATGTACAAGCTGATATAGCATATAACA
CAGAAATGCAAATCTGCTAATGAGTTGGACGGATCAACTCTCAATTCAAAAACATGTGTA
CTTCAAATGTATCCTGCAACAGTTCCAGCATCGGTTCTGAAAATTAATTCTGGAGCTACT
AAAAACCTAATCTGCAATGAACTCTCATATGTATATATTCAATGATATATTCAATTGTAA
ATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAG
AAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATATCCGCA
   
Sequence Name 53-K115894-0022-182-C12C-8409
GenBank Accession LN508258 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>53-K115894-0022-182-C12C-8409
ATTGAATATATCATTGAATATATACATATGAGAGTTCATTGCAGATTAGGTTTTTAGTAG
CTCCAGAATTAATTTTCAGAACCGATGCTGGAACTGTTGCAGGATACATTTGAAGTACAC
ATGTTTTTGAATTGAGAGTTGATCCGTCCAACTCATTAGCAGATTTGCATTTCTGTGTTA
TATGCTATATCAGCTTGTACATTTTATTCCTGATAGTTTCTTTTCATACCTATTTGGGGT
ACTTCCCTATCCTTCATAACGTAGATAAGTGTTTTCAGTTTTGAGCTTCAGATATGTGAT
TTGTTTTATATATAATCCAGAGTGGATTTCAATATAGATAAGTAAGCTCTGATACTTGGA
TTTCTGTTTGTAGTTTTACTTTTCGATTTTCCTAGGAGATCATGGAATCTTTCAGTTCCC
AATATATTGATCGATCTAATTAAGACACTTGACAATCTCTTTCCTATCACCAGAAAGGAG
AAGTACCTCGATCTTGTATTATATGCATTTATGTAGAGCGGA

Last Updated on 06.12.2018 16:11