Sequences from confirmation amplicon


Confirmation sequences for line 310B12 and hit At1g58807 are shown.
Line ID 310B12
Locus At1g58807 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:21781646
Primers used for PCR 25vs - 8474, show primer details
   
Sequence Name 38-K118077-0022-310-B12C-25vs
GenBank Accession LN503143 [GenBank]
Sequencing primer gene specific primer, 25vs: 5'-CCGAATGAAGCTAAATTCAGATAG-3'
Sequence (A. th genome BLAST matches underlined) 
>38-K118077-0022-310-B12C-25vs
ATGAGTTACCTCTGCATACTCTAAGCTCAAGTATCTTAAGTGGATGAGCTTTCCAATGCA
AGAAGCTAACTTCCCTCCTTTAATCTTCACTTCAATAAGATCTAACACCCTCAGAAGTTC
TAACCTTGTAAAGCTTGAACCTGCCAGGTTCCAACTTCCCAAGGTAACAACCACGAGAGC
TCGAAGTTTTGGATTGTTTATATCTTTCTCAACATGTAAAGTAGTAGGATATTGGTAGAC
AAACCTGCGAGATGTGACAGTAGACTGCAAGTTTGCAGTTGAAGGGCGGCTACTGGTAAT
TTGTAGGAAGTTTTCTTCTTTAGCTTTTAACAAACAAACTTCTCTCATCATGTCATGCAA
ATGACAAGTTTCAAATCTCGAAGTCTTTACATCTCTTTCGGAAATGACCATATTCCTCCT
CACCAGCTCCTCTATGTAGACATCTCCAACATCTCGAATGGTCTCTCCATCGTAATGCCT
AGGTTGGAATATTCCTTCTGCAGCCCAGTAATATGACAAGTTCTCTACCTTTATCTCATA
ATCTTCTGGAAAATGGGCCAAGTAGAGGAAACAATGCTTCAAATAACTTGGCAATTCTTC
AAAGCTTAGAGACAATACATTGTTACATGTATTGTTGTTGTCGTCATTAAAGTTAGTTCT
TCCTCCCACGAGATGAGATCCAATATTCTCAGATAATCTTCTCCAATCATGCGATGTGTA
TTTTTCAGCTAACATACCTCCTAACACTCTGATGGCCAATGGTAACCCTCCACAGTGTTT
GATCATTAGCTTACCCAACTCTTCCTTTTCCTCATCAATCTTAAATTCTGCACAACGTTA
TCAATATGTCAAAATCGTTTTACAAGTAGACTACAAAACCNAGATACTAAACAGCCAGAG
ATGCATTAAAAGTACCAGCTGCATCTTTCATAGGAAGTGCTATCCGCAGTATCCGGGAAA
TATTCAATTGTAAATGGCTTCATGTCCGGGGAATC
   
Sequence Name 13-K118076-0022-310-B12C-oriL
GenBank Accession LN492241 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>13-K118076-0022-310-B12C-oriL
TTGAATATTTCCCGGATACTGCGGATAGCACTTCCTATGAAAGATGCAGCTGGTACTTTT
AATGCATCTCTGGCTTGTTAGTATCTTGGTTTTGTAGTCTACTTGTAAAACGATTTTGAC
ATATTGATAACGTTGTGCAGAATTTAAGATTGATGAGGAAAAGGAAGAGTTGGGTAAGCT
AATGATCAAACACTGTGGAGGGTTACCATTGGCCATCAGAGTGTTAGGAGGTATGTTAGC
TGAAAAATACACATCGCATGATTGGAGAAGATTATCTGAGAATATTGGATCTCATCTCGT
GGGAGGAAGAACTAACTTTAATGACGACAACAACAATACATGTAACAATGTATTGTCTCT
AAGCTTTGAAGAATTGCCAAGTTATTTGAAGCATTGTTTCCTCTACTTGGCCCATTTTCC
AGAAGATTATGAGATAAAGGTAGAGAACTTGTCATATTACTGGGCTGCAGAAGGAATATT
CCAACCTAGGCATTACGATGGAGAGACCATTCGAGATGTTGGAGATGTCTACATAGAGGA
GCTGGTGAGGAGGAATATGGTCATTTCCGAAAGAGATGTAAAGACTTCGAGATTTGAAAC
TTGTCATTTGCATGACATGATGAGAGAAGTTTGTTTGTTAAAAGCTAAAGAAGAAAACTT
CCTACAAATTACCAGTAGCCGCCCTTCAACTGCAAACTTGCAGTCTACTGTCACATCTCG
CAGGTTTGTCTACCAATATCCTACTACTTTACATGTTGAGAAAGATATAAACAATCCAAA
ACTTCGAGCTCTCGTGGTTGTTACCTTGGGAAGTTGGAACCTGGCAGGTTCAAGCTTTAC
AAGGTTAGAACTTCTGAGGGTGTTAGATCTTATTGAAGTGAAGATTAAAGGAGGGAAGTT
AGCTTCTTGCATTGGAAAGCTCATCCACTAAGATACTTGAGCTTAGAGTATGCAGAGGGT
ACTCATATACCTTACTCA
Primers used for PCR 29vr - 8474, show primer details
   
Sequence Name 37-K118077-0022-310-B12C-29vr
GenBank Accession LN502775 [GenBank]
Sequencing primer gene specific primer, 29vr: 5'-GCAGCCCAGTAATATGACAAGTTC-3'
Sequence (A. th genome BLAST matches underlined) 
>37-K118077-0022-310-B12C-29vr
AGGAAACATGCTTCAATAACTTGGCAATTCTTCAAAGCTTAGAGACAATACATTGTTACA
TGTATTGTTGTTGTCGTCATTAAAGTTAGTTCTTCCTCCCACGAGATGAGATCCAATATT
CTCAGATAATCTTCTCCAATCATGCGATGTGTATTTTTCAGCTAACATACCTCCTAACAC
TCTGATGGCCAATGGTAACCCTCCACAGTGTTTGATCATTAGCTTACCCAACTCTTCCTT
TTCCTCATCAATCTTAAATTCTGCACAACGTTATCAATATGTCAAAATCGTTTTACAAGT
AGACTACAAAACCAAGATACTAACAAGCCAGAGATGCATTAAAAGTACCAGCTGCATCTT
TCATAGGAAGTGCTATCCGCAGTATCCGGGAAATATTCAATTGTAAATGGCTTCATGTCC
GGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTA
ATTACCAATTTTTTTTCAATTCAAAAATGTAGATATCCGC
   
Sequence Name 12-K118076-0022-310-B12C-8409
GenBank Accession LN491782 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>12-K118076-0022-310-B12C-8409
ATTTACATTGAATATTTCCCGGATACTGCGGATAGCACTTCCTATGAAAGATGCAGCTGG
TACTTTTAATGCATCTCTGGCTTGTTAGTATCTTGGTTTTGTAGTCTACTTGTAAAACGA
TTTTGACATATTGATAACGTTGTGCAGAATTTAAGATTGATGAGGAAAAGGAAGAGTTGG
GTAAGCTAATGATCAAACACTGTGGAGGGTTACCATTGGCCATCAGAGTGTTAGGAGGTA
TGTTAGCTGAAAAATACACATCGCATGATTGGAGAAGATTATCTGAGAATATTGGATCTC
ATCTCGTGGGAGGAAGAACTAACTTTAATGACGACAACAACAATACATGTAACAATGTAT
TGTCTCTAAGCTTTGAAGAATTGCCAAGTTATTTGAAGCATTGTTTCCTCTACTTGGCCC
ATTTTCCAGAAGATTATGAGATAAAGGTAGAGAACTTGTCATATATGGGGGCTG

Last Updated on 06.12.2018 16:11