Sequences from confirmation amplicon


Confirmation sequences for line 463H04 and hit At1g58807 are shown.
Line ID 463H04
Locus At1g58807 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:21781738
Primers used for PCR 25vt - 8474, show primer details
   
Sequence Name 36-K118278-0022-463-H04C-25vt
GenBank Accession LN502396 [GenBank]
Sequencing primer gene specific primer, 25vt: 5'-TCAATTAATTTGATGTTGAGCGTG-3'
Sequence (A. th genome BLAST matches underlined) 
>36-K118278-0022-463-H04C-25vt
AGCTGCTATTCTCTGTTGAGAAATTCTCTAAAGTCTCCAATTTTACTAGATTACTCAATT
CTAGTTTTGTCTTCCTCCCCATATCAGATGGTAACGCAAGGTATCTCAGTTCTTGCATCC
CCATCAAGACATTGGGCACAAATGTAGATCTTCCGAATGAAGCTAAATTCAGATAGATCA
ACAACTTCAGATTTCCTAGTGAGTAAGGTATATGAGTTACCTCTGCATACTCTAAGCTCA
AGTATCTTAAGTGGATGAGCTTTCCAATGCAAGAAGCTAACTTCCCTCCTTTAATCTTCA
CTTCAATAAGATCTAACACCCTCAGAAGTTCTAACCTTGTAAAGCTTGAACCTGCCAGGT
TCCAACTTCCCAAGGTAACAACCACGAGAGCTCGAAGTTTTGGATTGTTTATATCTTTCT
CAACATGTAAAGTAGTAGGATATTGGTAGACAAACCTGCGAGATGTGACAGTAGACTGCA
AGTTTGCAGTTGAAGGGCGGCTACTGGTAATTTGTAGGAAGTTTTCTTCTTTAGCTTTTA
ACAAACAAACTTCTCTCATCATGTCATGCAAATGACAAGTTTCAAATCTCGAAGTCTTTA
CATCTCTTTCGGAAATGACCATATTCCTCCTCACCAGCTCCTCTATGTAGACATCTCCAA
CATCTCGAATGGTCTCTCCATCGTAATGCCTAGGTTGGAATATTCCTTCTGCAGCCCAGT
AATATGACAAGTTCTCTACCTTTATCTCATAATCTTCTGGGAAATGGGCCAAGTAGAGGA
AACAATGCTTCAAATAACTTGGCAATTCTTCAAAGCTTAGAGACAATACATTGTTACATG
TATTGTTGTTGTCGTCATTAAAG
   
Sequence Name 04-K118277-0022-463-H04C-oriL
GenBank Accession LN488109 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>04-K118277-0022-463-H04C-oriL
TTTGACATATTGATAACGTTGTGCAGAATTTAAGATTGATGAGGAAAAGGAAGAGTTGGG
TAAGCTAATGATCAAACACTGTGGAGGGTTACCATTGGCCATCAGAGTGTTAGGAGGTAT
GTTAGCTGAAAAATACACATCGCATGATTGGAGAAGATTATCTGAGAATATTGGATCTCA
TCTCGTGGGAGGAAGAACTAACTTTAATGACGACAACAACAATACATGTAACAATGTATT
GTCTCTAAGCTTTGAAGAATTGCCAAGTTATTTGAAGCATTGTTTCCTCTACTTGGCCCA
TTTTCCAGAAGATTATGAGATAAAGGTAGAGAACTTGTCATATTACTGGGCTGCAGAAGG
AATATTCCAACCTAGGCATTACGATGGAGAGACCATTCGAGATGTTGGAGATGTCTACAT
AGAGGAGCTGGTGAGGAGGAATATGGTCATTTCCGAAAGAGATGTAAAGACTTCGAGATT
TGAAACTTGTCATTTGCATGACATGATGAGAGAAGTTTGTTTGTTAAAAGCTAAAGAAGA
AAACTTCCTACAAATTACCAGTAGCCGCCCTTCAACTGCAAACTTGCAGTCTACTGTCAC
ATCTCGCAGGTTTGTCTACCAATATCCTACTACTTTACATGTTGAGAAAGATATAAACAA
TCCAAAACTTCGAGCTCTCGTGGTTGTTACCTTGGGAAGTTGGAACCTGGCAGGTTCAAG
CTTTACAAGGTTAGAACTTCTGAGGGTGTTAGATCTTATTGAAGTGAAGATTAAAGGAGG
GAAGTTAGCTTCTTGCATTGGAAAGCTCATCCACTTAAGATACTTGAGCTTAGAGTATGC
AGAGGTAACTCATATACCTTACTCACTAGGAAATCTGAAGTTGTTGATCTATCTG

Last Updated on 06.12.2018 16:11