Sequences from confirmation amplicon


Confirmation sequences for line 200H10 and hit At1g62170 are shown.
Line ID 200H10
Locus At1g62170 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:22975100
Primers used for PCR BL90 - 8474, show primer details
   
Sequence Name 26-K105305-0022-200-H10C-BL90
GenBank Accession LN497923 [GenBank]
Sequencing primer gene specific primer, BL90: 5'-CCGGTTTGTTCTTCTCTAATCAAG-3'
Sequence (A. th genome BLAST matches underlined) 
>26-K105305-0022-200-H10C-BL90
CAAATCATAGGCTTCCCAAAGCGCAACCAAGGTATGCAGAACGAAATGCAGTAAAAGTAA
CAGCTTCGGTACCTTTTTCATCGATTTCAATCAAAGTTTTTTGGTAAAATGACACATCAA
GCTCAAAGTCACTGAAAGCACTTGAAGCTTCAAACCCGAATTCGATCTTAAACTTTGGAA
TTCTGAATTTACCAACTTTAACTCTTCTTTCTGGATTGTGACTATCCAAGAATCCAGGAG
TAGATGTCATTCTTTCCAAAAGATCATCCAATGAGTATGTAGATTTATATTATGAATTTG
CAATTGTAAAGGAAAAAACAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATC
TACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAA
TTTTTTTTCAATTCAAAAATGTAGATGTCCG
   
Sequence Name 10-K105306-0022-200-H10C-8409
GenBank Accession LN490649 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>10-K105306-0022-200-H10C-8409
ATTTACATTGAATATATCCTGTTTTTTCCTTTACAATTGCAAATTCATAATATAAATCTA
CATACTCATTGGATGATCTTTTGGAAAGAATGACATCTACTCCTGGATTCTTGGATAGTC
ACAATCCAGAAAGAAGAGTTAAAGTTGGTAAATTCAGAATTCCAAAGTTTAAGATCGAAT
TCGGGTTTGAAGCTTCAAGTGCTTTCAGTGACTTTGAGCTTGATGTGTCATTTTACCAAA
AAACTTTGATTGAAATCGATGAAAAAGGTACCGAAGCTGTTACTTTTACTGCATTTCGTT
CTGCATACCTTGGTTGCGCTTTGGTGAAGCCTATTGATTTTGTAGCAGATCATCCATTTC
TTTTCTTGATTAGAGAAGAACAAACCGG

Last Updated on 06.12.2018 16:11