Sequences from confirmation amplicon


Confirmation sequences for line 159E04 and hit At1g62330 are shown.
Line ID 159E04
Locus At1g62330 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:23049394
Primers used for PCR 12xc - 8474, show primer details
   
Sequence Name 43-K119110-0022-159-E04C-12xc
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer gene specific primer, 12xc: 5'-GTCCATTTCTTAGGATTCGGAAA-3'
Sequence (A. th genome BLAST matches underlined) 
>43-K119110-0022-159-E04C-12xc
GTAAAGAACCTTCTTTTAGCACCGTGGATTCGATATATTACCCGTAATGATCCAACTTTT
TCTTGGGGAGTAGAGGCTGCGGTGTAGATGTAATTTTCATGCGCTAAACTTCGTTCCGAA
GATACAAGAAACGGGTGCGATACTCGTAAGGAGATTACGAGATAGTGGATCACACCTTGC
ACCAGTTGACCCGTATCTCGTCGGTCCAAAATTTGCGTCATTCATATTGGACAAGAAAGC
AGGTCCTCTCCATAAGGCTTCTAAGTATCTAGCAGTCCATCTCAGGTTTGAAATTGATAT
GGTGGCTCATTCTCTCTGTTACTTCGGAGGAGGTGATGCGGAAAAGGCAGAGCTTGATGC
TTACCGGGAAAAGCATTTCCCGACGTTGGCAAATCTAACAAAGACGCAAAAGTTTGTTCC
TTTCCTCTCTTAACTCTTTTTCACATTATTATTTCAGTTTTGGTATCTCACTGAATGCCC
TTTTGTTTTCTCTTATGCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCT
ACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAAT
TTTTTTTCAATTCANAAATGAAGATATCCGCAGCGTTTATT
   
Sequence Name 20-K119109-0022-159-E04C-8409
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>20-K119109-0022-159-E04C-8409
TCATTGAATATATCCTGCATAAGAGAAAACAAAAGGGCATTCAGTGAGATACCAAAACTG
AAATAATAATGTGAAAAAGAGTTAAGAGAGGAAAGGAACAAACTTTTGCGTCTTTGTTAG
ATTTGCCAACGTCGGGAAATGCTTTTCCCGGTAAGCATCAAGCTCTGCCTTTTCCGCATC
ACCTCCTCCGAAGTAACAGAGAGAATGAGCCACCATATCAATTTCAAACCTGAGATGGAC
TGCTAGATACTTAGAAGCCTTATGGAGAGGACCTGCTTTCTTGTCCAATATGAATGACGC
AAATTTTGGACCGACGAGATACGGGTCAACTGGTGCAAGGTGTGATCCACTATCTCGTAA
TCTCCTTACGAGTATCGCACCCGTTTCTTGTATCTTCGGAACGAAGTTTAGCGCATGAAA
ATTACATCTACACCGCAGCCTCTACTCCCCAAGAAAAAGTTGGATCATTACGGGTAATAT
ATCGATATCCACGGTGCTAAAAGAAGGTTCTTTACCTGTAACTCANACGGTATCGGGTCA
AAGGCCAAACGGTTTCCGAATCCTAAGAAATGGA

Last Updated on 06.12.2018 16:11