Sequences from confirmation amplicon


Confirmation sequences for line 641F12 and hit At1g62660 are shown.
Line ID 641F12
Locus At1g62660 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:23202995
Primers used for PCR 20vd - 8474, show primer details
   
Sequence Name 23-K117753-0022-641-F12C-20vd
GenBank Accession LN496733 [GenBank]
Sequencing primer gene specific primer, 20vd: 5'-TGGTCTTCTGTTCAGGTACCTTTT-3'
Sequence (A. th genome BLAST matches underlined) 
>23-K117753-0022-641-F12C-20vd
ACCCGAGTTAGGGCTTAACTTTTTTTTGTAACCATGATCAGGGCATCCCAAGAACTGTTG
TATTGGACACAAGGACGCATAAAAACTTAGTCCAGTGGCCAGTTGAGGAAATCAAATCAT
TGAGACTAAGCAGCAAGAAATTTGACATGACTATTGGACCAGGGACTGTGGTTCCGGTCG
ATGTGGGTTCCGCCACTCAGCTAGACATAGAGGCTGAGTTCGAGATCAAGACCGATGATC
TCAAGTTATTCTTTGATGATGACTCTGTGGAGGCCGACAATAAATTCAGCTGCGAAACAA
ACGGAGGCTCCACAGCGCGTGGTGCTTTAGGGCCTTTTGGATTCTCGGTTCTCGCTGACG
AGGGCTTGTCAGAACAAACTCCGGTTTACTTCTATGTGACTAAGGGAAATATTCAATTGT
AAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGG
AGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATATCCCCGCTT
TTTTT
   
Sequence Name 07-K117752-0022-641-F12C-8409
GenBank Accession LN489457 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>07-K117752-0022-641-F12C-8409
TTGAAATTTCCCTTAGTCACATAGAAGTAAACCGGAGTTTGTTCTGACAAGCCCTCGTCA
GCGAGAACCGAGAATCCAAAAGGCCCTAAAGCACCACGCGCTGTGGAGCCTCCGTTTGTT
TCGCAGCTGAATTTATTGTCGGCCTCCACAGAGTCATCATCAAAGAATAACTTGAGATCA
TCGGTCTTGATCTCGAACTCAGCCTCTATGTCTAGCTGAGTGGCGGAACCCACATCGACC
GGAACCACAGTCCCTGGTCCAATAGTCATGTCAAATTTCTTGCTGCTTAGTCTCAATGAT
TTGATTTCCTCAACTGGCCACTGGACTAAGTTTTTATGCGTCCTTGTGTCCAATACAACA
GTTCTTGGGATGCCCTGATCATGGTTACAAAAAAAAGTTAAGCCCTAACTCGGGTAACAA
GTAACTCAAAGACCTAACGTTCATAGCACGCTACGTGAAGAAAAGGTACCTGAC

Last Updated on 06.12.2018 16:11