Sequences from confirmation amplicon


Confirmation sequences for line 426C02 and hit At1g63140 are shown.
Line ID 426C02
Locus At1g63140 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:23418253
Primers used for PCR 14vi - 8474, show primer details
   
Sequence Name 42-K117953-0022-426-C02C-14vi
GenBank Accession LN504627 [GenBank]
Sequencing primer gene specific primer, 14vi: 5'-ACATTGGAAGTAGAAAGACCGAGA-3'
Sequence (A. th genome BLAST matches underlined) 
>42-K117953-0022-426-C02C-14vi
CGGTTGCAGTTTTTCTTGACCGCGGAGATGGCTTGGGCTCTCTCGCCACTTTGTTCATGG
TACTCCAAGGGGAAGTCTGTATGAAGCCTTGGTACGATCCTAGTTCATTATTCACAAAAT
GTTCTACATATATACACGTAACGTTAACTAAAACTGATTCTAATTTATTTTTACTAGGGA
ACATCTCAAAGACATGATATTAGAAGGAAAAGATGCATTCACCTCTGCTCATGGCATGAG
GTTTTTCGAACTCATTGGTTCGAACGAACAATTCGCTGAAATGTTTAACCGGGCAATGTC
GGAAGCTTCCACATTGATTATGAAGAAGGTTTTAGAAGTTTACAAAGGATTCGAAGATCA
AGTGACTTCAATATGTAAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGC
AATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATT
CAAAAATGTAGATGTCCGCAGCGTTATTAT
   
Sequence Name 18-K117952-0022-426-C02C-8409
GenBank Accession LN494497 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>18-K117952-0022-426-C02C-8409
CATTTACAATTTACTATTGAAGTCACTTGATCTTCGAATCCTTTGTAAACTTCTAAAACC
TTCTTCATAATCAATGTGGAAGCTTCCGACATTGCCCGGTTAAACATTTCAGCGAATTGT
TCGTTCGAACCAATGAGTTCGAAAAACCTCATGCCATGAGCAGAGGTGAATGCATCTTTT
CCTTCTAATATCATGTCTTTGAGATGTTCCCTAGTAAAAATAAATTAGAATCAGTTTTAG
TTAACGTTACGTGTATATATGTAGAACATTTTGTGAATAATGAACTAGGATCGTACCAAG
GCTTCATACAGACTTCCCCTTGGAGTACCATGAACAAAGTGGCGAGAGAGCCCAAGCCAT
CTCCGCGGTTCAAGAAAAACGTGCAAACCGGTTCAGCTGCATAGACCCTCTCGGTCTTTC
TACTTCCAATGT

Last Updated on 06.12.2018 16:11