Sequences from confirmation amplicon


Confirmation sequences for line 014H04 and hit At1g66130 are shown.
Line ID 014H04
Locus At1g66130 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:24615504
Primers used for PCR 21lj - 3144, show primer details
   
Sequence Name 39-K113916-0022-014-H04C-21lj
GenBank Accession LN503425 [GenBank]
Sequencing primer gene specific primer, 21lj: 5'-GTCAGTCTTTCCAATACTTGCTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>39-K113916-0022-014-H04C-21lj
AATAGTTTAATATAACTTAAACTAAGTGACGAAACCTATCGTTCGTTATCATTCGAAACA
ACATCTTACACGTTGAACACCTTAAAAACTCTACTCTAGTTCTAGTACAATTGAAAGGTT
TTATGTGTTATTCTAACTTATATGTGGGGCAATAAATAAAGTCAAAGATTCTATGGACCA
ATTTTGCAATTTGAATAAAAAGGCAGAGCGCTTACGTGGTATCAATTAAATGAAATGATA
GTTTCGTGGTACACTGATAGTTTAAACCGAAGGCGGGAAACGACAATCTGATCCCCGGGT
ACCCTGTCCTCTCCAAATGAAATGAACTTCCTTATATAGAGGAAGGGTCTTGCGAAGGAT
AGTGGGATTGTGCGTCATCCCTTACGTCAGTGGAGATTC
   
Sequence Name 11-K113915-0022-014-H04C-3144
GenBank Accession LN491198 [GenBank]
Sequencing primer T-DNA primer, 35St: 5'-GTGGATTGATGTGATATCTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>11-K113915-0022-014-H04C-3144
TTCATTTCATTTTGGAGAGGATCGGGTACCCGGGGATCAGATTGTCGTTTCCCGCCTTCG
GTTTAAACTATCAGTGTACCACGAAACTATCATTTCATTTAATTGATACCACGTAAGCGC
TCTGCCTTTTTATTCAAATTGCAAAATTGGTCCATAGAATCTTTGACTTTATTTATTGCC
CCACATATAAGTTAGAATAACACATAAAACCTTTCAATTGTACTAGAACTAGAGTAGAGT
TTTTAAGGTGTTCAACGTGTAAGATGTTGTTTCGAATGATAACGAACGATAGGTTTCGTC
ACTTAGTTTAAGTTATATTAAACTATTCATGAGCATACGTTGAAGGATTGAATCTAATCT
ATAATGTTTCACTTATCATTACGCCTAAAGTATATATGAAACATTCTCTGACACATGTTG
CAGATGTATAGCACGATGACCACTCCGGTACCGGAGCAAGT

Last Updated on 06.12.2018 16:11