Sequences from confirmation amplicon


Confirmation sequences for line 751D11 and hit At1g67800 are shown.
Line ID 751D11
Locus At1g67800 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:25423667
Primers used for PCR 05uc - 8474, show primer details
   
Sequence Name 32-K117118-0022-751-D11C-05uc
GenBank Accession LN500737 [GenBank]
Sequencing primer gene specific primer, 05uc: 5'-CTTCTTATCAAAATCAAACACGCA-3'
Sequence (A. th genome BLAST matches underlined) 
>32-K117118-0022-751-D11C-05uc
ATCCAAGTTTGGATTTTTTCATCAAGTCTTCACCTTTTACATTGTTTAAACACGTTTGAC
TCGGTAAATTTCACATTCATTGCCTAATAAACTAATATATATCAGTAAGTCGCTTTCAGG
AGGATTATAGCATCGCCGTGAAATACAAGGAAATTCTCAAAGTTTTTCCGGCGAGAAATG
ATAAATTAAGGGAAGACCCAATTCGGAAAATTACAGGAAACAAGGGCTTTACGAGTTGAC
TGGTTCAAACTAATTGAGAGAGTCACAGAGTAATTACAGGATATATTCAATTGTAAATGG
CTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAA
AGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCACGATTATTA
T
   
Sequence Name 10-K117117-0022-751-D11C-8409
GenBank Accession LN490835 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>10-K117117-0022-751-D11C-8409
ATCCTGTAATTACTCTGTGACTCTCTCAATTAGTTTGAACCAGTCAACTCGTAAAGCCCT
TGTTTCCTGTAATTTTCCGAATTGGGTCTTCCCTTAATTTATCATTTCTCGCCGGAAAAA
CTTTGAGAATTTCCTTGTATTTCACGGCGATGCTATAATCCTCCTGAAAGCGACTTACTG
ATATATATTAGTTTATTAGGCAATGAATGTGAAATTTACCGAGTCAAACGTGTTTAAACA
ATGTAAAAGGTGAAGACTTTGATGAAAAAATCCAAACTTTGGATCTGTTTCAGAGTCAAT
AAGCTCTCTCATATTTATATATGCGTGTTTGATTTGGATAAGAAG

Last Updated on 06.12.2018 16:11