Sequences from confirmation amplicon


Confirmation sequences for line 804G08 and hit At1g68530 are shown.
Line ID 804G08
Locus At1g68530 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:25713613
Primers used for PCR KU11 - 8474, show primer details
   
Sequence Name 59-K102176-0022-804-G08C-8409
GenBank Accession LN509957 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>59-K102176-0022-804-G08C-8409
TTACAATTGAATATATCCTGATAATATCTTGATATAGTTTTCACCGTGTATATCATTGTT
AGTGTATATATGTAGGTGAAATAAAATGATGTGTGTTTATTACCTATTGTGGTGATGTTT
GCCTTGAGGGCTTCACCGGCGATGGCCATGAGATCTTTGGACAAGTTGATGCCAACGTGT
CCTTCTTTGTCTTCCTGTTCGTAGACACAGTAGAAAGACTTGTCGTCAGCGCCACGGTGT
GTCCGGACGAGGTGGGAAAGCTTGTATTTGGCTCGCCACCGGTCAGACCGGCGGTTTGAC
ATGTGTATGGCTGCCGCACCCATGCGGAAGAGACAATTGGGTAACAACATGGCTCTCTCG
TTGCCTTGATAGTAATTAGGCGT
   
Sequence Name 59-K102180-0022-804-G08C-KU11
GenBank Accession LN509958 [GenBank]
Sequencing primer gene specific primer, KU11: 5'-AACGCCTAATTACTATCAAGGCAA-3'
Sequence (A. th genome BLAST matches underlined) 
>59-K102180-0022-804-G08C-KU11
CTCTTCCGCATGGGTGCGGCAGCCATACACATGTCAAACCGCCGGTCTGACCGGTGGCGA
GCCAAATACAAGCTTTCCCACCTCGTCCGGACACACCGTGGCGCTGACGACAAGTCTTTC
TACTGTGTCTACGAACAGGAAGACAAAGAAGGACACGTTGGCATCAACTTGTCCAAAGAT
CTCATGGCCATCGCCGGTGAAGCCCTCAAGGCAAACATCACCACAATAGGTAATAAACAC
ACATCATTTTATTTCACCTACATATATACACTAACAATGATATACACGGTGAAAACTATA
TCAAGATATTATCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATG
GATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTT
TTCAATTCAAAAATGTAGATGTCCGCA

Last Updated on 06.12.2018 16:11