Sequences from confirmation amplicon


Confirmation sequences for line 838H09 and hit At1g68540 are shown.
Line ID 838H09
Locus At1g68540 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:25719983
Primers used for PCR 18do - 8474, show primer details
   
Sequence Name 29-K110172-0022-838-H09C-18do
GenBank Accession LN499334 [GenBank]
Sequencing primer gene specific primer, 18do: 5'-TCCTTTTCAATGTGGCTTTAGACT-3'
Sequence (A. th genome BLAST matches underlined) 
>29-K110172-0022-838-H09C-18do
TTTGTTGTACCTTTATATAGGATCACCAAAGTCTCCTGAATGTTATGATCCTGTGGAATA
AGAACAGGAGAAGCAGTGTGGAATACTCCATCAACACCATTCACTGCTTCATCAAAACTT
CCTTCTACCGTTAGATCAGCTTGGAGAATCTTTAGCCTCTGTTTTGCTCCTTGAAATTCC
CATAAAAATCCAACCTTTTCTTCATCACCTTTCAATACAAATACCAAATCCAAAATCAGG
AGATTAAAGGTGGAAACTTTGAATGATTCTAGTGTGAGAGAATTGAGAAATTACGTGGGT
TTCGGACAGTTGTTCGAACGGTGTGTCCGAGTTCGAGAAGTGATTTGATGATGTAAGAAG
CAATGAAACCTGTTCCTCCAGTTACCAAATACTCAGACATCTTTGTCTCTGCAACGAATG
TGATATTGAGATTGTGATTTGGTTTAATAAAAGTGAGCTTTGTGGATGTAAAAGCAGAGT
GGGAAATCAATTATACATGGAAACCTAAGCACATATATATATGTGTAATCTTTGCCATTT
TTCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAAT
GAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAA
AAATGTAGATGTCCGCAGCGTTA
   
Sequence Name 13-K110171-0022-838-H09C-8409
GenBank Accession LN492086 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>13-K110171-0022-838-H09C-8409
ATCGGACATGAGCCATTTACAATTGAATATATCCTGAAAAATGGCAAAGATTACACATAT
ATATATGTGCTTAGGTTTCCATGTATAATTGATTTCCCACTCTGCTTTTACATCCACAAA
GCTCACTTTTATTAAACCAAATCACAATCTCAATATCACATTCGTTGCAGAGACAAAGAT
GTCTGAGTATTTGGTAACTGGAGGAACAGGTTTCATTGCTTCTTACATCATCAAATCACT
TCTCGAACTCGGACACACCGTTCGAACAACTGTCCGAAACCCACGTAATTTCTCAATTCT
CTCACACTAGAATCATTCAAAGTTTCCACCTTTAATCTCCTGATTTTGGATTTGGTATTT
GTATTGAAAGGTGATGAAGAAAAGGTTGGATTTTTATGGGAATTTCAAGGAGCAAAACAG
AGGCTAAAGATTCTCCAAGCTGATCTAACGGTAGAAGGAAGTTTTGATGAAGCAGTGAAT
GGTGTTGATGGAGTATTCCACACTGCTTCTCCTGTTCTTGTTCCACAGGATCATAACATT
CAGGAGACTTTGGTTGATCCTATTATAAAGGGTACAACAAATGTGATGAGCTCTTGTGCC
AAGTCTAAAGCCACATGNAAAAAG

Last Updated on 06.12.2018 16:11