Sequences from confirmation amplicon


Confirmation sequences for line 418B11 and hit At1g69120 are shown.
Line ID 418B11
Locus At1g69120 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:25983463
Primers used for PCR BC02 - 8474, show primer details
   
Sequence Name 33-K103981-0022-418-B11C-BC02
GenBank Accession LN500969 [GenBank]
Sequencing primer gene specific primer, BC02: 5'-TAGACCAACAACAACAAAAAGTCG-3'
Sequence (A. th genome BLAST matches underlined) 
>33-K103981-0022-418-B11C-BC02
CACATTCATGTAATTTTCCACACTAATAAGTTTGTTCGTTTGGCGACCATTGCCTTAACC
TACGTTAATAAAATAATGTTTTAAAATAAAGTTTTCTAGTTTCAAAATCATGACATCATG
TAACCATCACTAACAGCTATAACTTCAATGAAATTACAACACCTAACGTAACTAGCTTGC
ATAACATATGTGCTTTAAATTAAGACTGATTAGTAATTTTTTGTTACCCCATGTTGAGAA
AAGGAGATGGCTGATGAGAGAGCATGTAAGGATGCTGGATTTGGTGCTGCTGCGGTGGCA
GAGGGGGAGGCATATTGTGGCCTTGGTTCTGCTGATCCCACTGCTCCTGTTGAGCCCTAA
GAATTTTTTCCCTCTCCTTGATCTGCCAGCAAATTTATTTCAAACCATATTATCATCATC
AATTTTCAAAGTGAAGAAACTATATATATGCAATGGTTACAAATAGTTATTTTCACAGTT
GCACTCATGCAATGAACTATTATAACATTTTTATAATTAGGAGTAGTGTCTATATTCATA
TACATAGATATGAGCTAAGTAGAATGATGGGACAACATTTCAAAGTTTCAAGAAGAAAAT
TATTATATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAA
AAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCC
   
Sequence Name 49-K103982-0022-418-B11C-8409
GenBank Accession LN506820 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>49-K103982-0022-418-B11C-8409
ATTTTCTTCTTGAACTTTGAAATGTTGTCCCATCATTCTACTTAGCTCATATCTATGTAT
ATGAATATAGACACTACTCCTAATTATAAAAATGTTATAATAGTTCATTGCATGAGTGCA
ACTGTGAAAATAACTATTTGTAACCATTGCATATATATAGTTTCTTCACTTTGAAAATTG
ATGATGATAATATGGTTTGAAATAAATTTGCTGGCAGATCAAGGAGAGGGAAAAAATTCT
TAGGGCTCAACAGGAGCAGTGGGATCAGCAGAACCAAGGCCACAATATGCCTCCCCCTCT
GCCACCGCAGCAGCACCAAATCCAGCATCCTTACATGCTCTCTCATCAGCCATCTCCTTT
TCTCAACATGGGGTAACAAAAAATTACTAATCAGTCTTAATTTAAAGCACATATGTTATG
CAAGCTAGTTACGTTAGGTGTTGTAATTTCATTGAAGTTATAGCTGTTAGTGATGGTTAC
ATGATGTCATGATTTGAAACTANGAAACTTTATTTTAAACATTATT

Last Updated on 06.12.2018 16:11