Sequences from confirmation amplicon


Confirmation sequences for line 138D05 and hit At1g69190 are shown.
Line ID 138D05
Locus At1g69190 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:26013209
Primers used for PCR BU21 - 8474, show primer details
   
Sequence Name 67-K104377-0022-138-D05C-BU21
GenBank Accession LN511807 [GenBank]
Sequencing primer gene specific primer, BU21: 5'-TAGACGAGAACATGCATAAGGTTG-3'
Sequence (A. th genome BLAST matches underlined) 
>67-K104377-0022-138-D05C-BU21
GATCTGCCATGAGAGGAGATCCATGTACAATGCAAAACAAAGAGAATTTGGAGTATAATG
AAATATGCAAAGATGTTGCCACAGAGCTTTATGAGAGAGTAAGAGAAGCAGAACTCTCTG
GGATTCCAGCTTGGAGGATTATGATTGATCCAGGAATTGGATTTTCTAAGGGAATAGATC
ATAATTTAGATATTGTCATGGAGCTACCAAAAATCAGGGAAGAGATGGCTAAGAAGAGTA
TAGGATTGTCTCATGCACCTATACTTATAGGACCTTCAAGGAAGAGATTTTTGGGAGATA
TTTGTGGTCGTCCTGAGGCAAGTGAAAGAGATGCTGCAACTGTTGCTTGTGTTACTGCTG
GGATAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATA
TGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCA
CGGTTTATTA
   
Sequence Name 83-K104378-0022-138-D05C-8409
GenBank Accession LN514310 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>83-K104378-0022-138-D05C-8409
TCCAGCAGTAACACAAGCAACAGTTGCAGCATCTCTTTCACTTGCCTCAGGACGACCACA
AATATCTCCCAAAAATCTCTTCCTTGAAGGTCCTATAAGTATAGGTGCATGAGACAATCC
TATACTCTTCTTAGCCATCTCTTCCCTGATTTTTGGTAGCTCCATGACAATATCTAAATT
ATGATCTATTCCCTTAGAAAATCCAATTCCTGGATCAATCATAATCCTCCAAGCTGGAAT
CCCAGAGAGTTCTGCTTCTCTTACTCTCTCATAAAGCTCTGTGGCAACATCTTTGCATAT
TTCATTATACTCCAAATTCTCTTTGTTTTGCATTGTACATGGATCTCCTCTCATGTGCAT
GATCATATAAGGAACATCAGAATCCGCAACAACCTTATGCATGTTCTCGTC
Primers used for PCR CB41 - 8474, show primer details
   
Sequence Name 36-K118837-0022-138-D05C-CB41
GenBank Accession LN502417 [GenBank]
Sequencing primer gene specific primer, CB41: 5'-TGTCATAAATTTGATTATGCCCAA-3'
Sequence (A. th genome BLAST matches underlined) 
>36-K118837-0022-138-D05C-CB41
GTAAATGAAATCTAAAAATATCACAAATGAGTACTTTTTATGTGTACTTTGTGAGTTAAG
TCCCAATACGAAAATGAATCATAATGTACAGTATCNGGGCTTTTTATGACAAGAGATCCT
ACTAAACTCTTGTGGGCTGAATACAAAGGCTAGCAAATAAGACTTAAAAGAAACTTTACA
ACTGCGAATAGACAAAGGGATTTTAAAACTGCCAATAAACTTTATAAAATGAACTAAATT
CTTCTGAATTTCATACGGAAAAACAAAATAAACTTATTTTTAACACATCTCAAGTCTTAA
AACCATACAACAAATCATATCAACTAATCAACATTTTTGAACCTTTTCGTCATCATTGCA
TCGCATAGCCTTGCCGCGTCTACATTATCTCTAACATTATGATAGGAAATATATTCAATT
GTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATAT
GGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAC
GGTTTATT
   
Sequence Name 12-K118836-0022-138-D05C-8409
GenBank Accession LN491808 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>12-K118836-0022-138-D05C-8409
TTGAATATATTTCCTATCATAATGTTAGAGATAATGTAGACGCGGCAAGGCTATGCGATG
CAATGATGACGAAAAGGTTCAAAAATGTTGATTAGTTGATATGATTTGTTGTATGGTTTT
AAGACTTGAGATGTGTTAAAAATAAGTTTATTTTGTTTTTCCGTATGAAATTCAGAAGAA
TTTAGTTCATTTTATAAAGTTTATTGGCAGTTTTAAAATCCCTTTGTCTATTCGCAGTTG
TAAAGTTTCTTTTAAGTCTTATTTGCTAGCCTTTGTATTCAGCCCACAAGAGTTTAGTAG
GATCTCTTGTCATAAAAAGCCCGATACTGTACATTATGATTCATTTTCGTATTGGGACTT
AACTCACAAAGTACACATAAAAAGTACTCATTTGTGATATTTTTAGATTTCATTTTACCT
GTCATATCGGTATTAACAACATTTGAGTATTTTGGGCATAATCAATNTTATGAC

Last Updated on 06.12.2018 16:11