Sequences from confirmation amplicon


Confirmation sequences for line 345C10 and hit At1g70460 are shown.
Line ID 345C10
Locus At1g70460 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:26557685
Primers used for PCR DL78 - 8474, show primer details
   
Sequence Name 54-K018604-022-345-C10C-DL78
GenBank Accession LN508333 [GenBank]
Sequencing primer gene specific primer, DL78: 5'-AGCTTTCCACTTTGTGCATATTCT-3'
Sequence (A. th genome BLAST matches underlined) 
>54-K018604-022-345-C10C-DL78
AAGTACCTGATAACAAAAGCCAACAAAATAGAGGCTTAGGTACTTCTCAGATGTTATTAA
TATATGGCAATAAGTAATACCTTCTAACCTTTGTATACAAGCAAACTAGTTCATCCTTAT
CATATCTAGTAGTTTAATAAAGAATTTGTGATCAATCTTACCCGAAGGTTCCCATAACGC
GAGTTGATACATGAGTTTGTGTTGAATCATTGAGTTTGGCAAGTCCAAAGTCAGCAACCT
ACACACCAAAATAAACAGAAAACTAAGAACAAATAAACTCTCATTAACTAAATCTACAGA
GCCAAAAAAAACTCCAAAAAAGAGCAAATGTTCAAAGAAAAAAAGGATTGCCTGAGCTTC
AAACTCATCGTCCAGCAAGATGTTAGCGGACTTTATGTCCCTGTGAATGATTTTTGGGTG
ACCTGCAAAACAAAACAAAAACATACATAAACAAATTAAGTTGCTTTGATTCAGCTTCCG
TTTGAACTTTGAAATGCATAAACATTGCAAAAGTACTTACAGTCTTCGTGTAAATACGCC
AAACCTTTGGCAGAACCTACATGGCTTCATGTACTTACAGGATATATTCAATTGTAAATG
GCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAANTATGGAGAA
NAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTAT
TATTAAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATTGGCG
AAAGCAATAAACAAATTATT
   
Sequence Name 55-K018604-022-345-C10C-8409
GenBank Accession LN508661 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>55-K018604-022-345-C10C-8409
GTAGATTTCCCGGACATGAAGCCATTTACAATTGAATATATCCTGTAAGTACATGAAGCC
ATGTAGGTTCTGCCAAAGGTTTGGCGTATTTACACGAAGACTGTAAGTACTTTTGCAATG
TTTATGCATTTCAAAGTTCAAACGGAAGCTGAATCAAAGCAACTTAATTTGTTTATGTAT
GTTTTTGTTTTGTTTTGCAGGTCACCCAAAAATCATTCACAGGGACATAAAGTCCGCTAA
CATCTTGCTGGACGATGAGTTTGAAGCTCAGGCAATCCTTTTTTTCTTTGAACATTTGCT
CTTTTTTGGAGTTTTTTTTGGCTCTGTAGATTTAGTTAATGAGAGTTTATTTGTTCTTAG
TTTTCTGTTTATTTTGGTGTGTAGGTTGCTGACTTTGGACTTGCCAAACTCAATGATTCA
ACACAAACTCATGTATCAACTCGCGTTATGGGAACCTTCGGGTAAGATTGATCACAAATT
CTTTATTAAACTACTAGATATGATAAGGATGAACTAGTTTGCTTGTATACAAAGGTTAGA
AGGTATTACTTATTGCCATATATTAATAACATCTGAGAAGTACCTAAGCCTCTATTTTGT
TGGCTTTTGTTATCAGGTACTTGGCACCAGAATATGCA

Last Updated on 06.12.2018 16:11