Sequences from confirmation amplicon


Confirmation sequences for line 015E03 and hit At1g71750 are shown.
Line ID 015E03
Locus At1g71750 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:26988718
Primers used for PCR RZ08 - 8474, show primer details
   
Sequence Name 03-K019977-022-015-E03C-RZ08
GenBank Accession LN487288 [GenBank]
Sequencing primer gene specific primer, RZ08: 5'-ACGAAGCTCCTTAGCCTTATAACC-3'
Sequence (A. th genome BLAST matches underlined) 
>03-K019977-022-015-E03C-RZ08
CACCTTTTTCACTGATTGCTTCTGATTGCATATCACACAAACCCACTTGTTACTACTTTT
CTTCTTCTGCTTCACCTGCGAATTCGAACCCCAAAACAAAATCCCTAAAAACTAACCAAT
ATATAAATTGACAGAGATAGAAGAGGAGGGAGCGAGAGAGTTTAATACCTGCATAGTTGA
ACACTCGTAACATTGGAGAGCGATGAAGAGAGTACTCGCCATTTTTTCTCCTTCTCCTTC
TTCGTCTTCGTCGTCTGCTCTCTAGGGATTTCGATTTTGTGTTTTCGATTTCGTTCTAGC
GCCAATTTGATATACTTCAAAAAATTTGGCAATTCCAAATTATTTTGTACCGGGTCTGTT
TAACAGAAGACGGTAAATAGTTTCGGTTAGTTTCGGTTAAGCTCCCGCGTTTTTTTACAA
TAGCAATTAGAATCAAACCCTAAAAACCCAAATCAAAAAGCTTCGTTGTGGAGGTAAGTA
GGAGGAAGACGATGGCGTTAGAGAAGCACATAGAGAAGGTACTCTTCAGCGATGAAGTAA
TTGCGCACCGGGTGAATCAGCTTGGAATCGATATTACTTCTGATTTCTCCGGTGACTCGG
AGGAAACTCCTATTTTTGTCGGCGTAGCCACTGGTGCTTGCCTCTTCTTGGCCGATCTCG
TTAGGCGAATTGACTTGCCAATAGCTATAGATTTCATTAGAGCTGAGTCTTACGGCTCTG
GTACTGTATCCAGTGGAGTTCCCAGAGTATCATTTGACTTAAAGCTTGACAT
   
Sequence Name 07-K019977-022-015-E03C-8409
GenBank Accession LN489059 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>07-K019977-022-015-E03C-8409
CTCAAACTTCATTACATTCAAATGAATACTATAACTCAATTGCTAAAGAAACTGAATCAA
AACTGATGCAAAACACATATTACCCAAATCCAGATCCATCACGCATAAAGAGATGGAGAT
TACCAAGACAACGTGCTTGTTCGTGATGTCAAGCTTTAAGTCAAATGATACTCTGGGAAC
TCCACTGGATACAGTACCAGAGCCGTAAGACTCAGCTCTAATGAAATCTATAGCTATTGG
CAAGTCAATTCGCCTAACGAGATCGGCCAAGAAGAGGCAAGCACCAGTGGCTACGCCGAC
AAAAATAGGAGTTTCCTCCGAGTCACCGGAGAAATCAGAAGTAATATCGATTCCAAGCTG
ATTCACCCGGTGCGCAATTACTTCATCGCTGAAGAGTACCTTCTCTATGTGCTTCTCTAA
CGCCATCGTCTTCCTCCTACTTACCTCCACAACGAAGCTTTTTGATTTGGGTTTTTAGGG
TTTGATTCTAATTGCTATTGTAAAAAAACGCGGGAGCTTAACCGAAACTAACCGAAACTA
TTTACCGTCTTCTGTTAAACAGACCCGGTACAAAATAATTTGGAATTGCCAAATTTTTTG
AAGTATATCAAATTGGCGCTAGAACGAAATCGAAAACACAAAATCGAAATCCCTAGAGAG
CAGACGACGAAGACGAAGAAGGAGAAGGAGAAAAAATGGCGAGTACTCTCTTCATCGCTC
TCCAATGT
Primers used for PCR 52wn - 8474, show primer details
   
Sequence Name 45-K118466-0022-015-E03C-52wn
GenBank Accession LN505687 [GenBank]
Sequencing primer gene specific primer, 52wn: 5'-CCAATGTAAGATAGGTTGCGGTAT-3'
Sequence (A. th genome BLAST matches underlined) 
>45-K118466-0022-015-E03C-52wn
GAACTGCAACATATTCAGAAGATACAACTCATGTTCAACACAAAGCCATGAAATATATAT
TCTTAATTCAGGCTAGTTCCTAACACCAACAAATACTTTTGTTTACCATGGTTTAGCTAA
GAAAAGGCATAACAGGGCAAATAAGAACGGAAAGCATAATGTCATGGTCTCACCTATTCT
ATAAATAGCTGTATACTCTGGTTAAGGTGGCTTAAACATTCCAAAGGCCTTAGACAGACG
TATAATGATAATACATATATTGAACAGAGAGAAACAAGAAACAGCTTACTTCAAAACCAC
TGTAGAATTTCCCCTTTCCAACCAGCTTATAATGAACCTTTCTTCTCGATGGCTTGTCGA
GGAGAGTGCAAACCGAAACAGATGACGCCTTTTTTGCTTTCATGTGCTCAATCAGGCAGC
TAAGTGTATTGCCAGTATCCACAATGTCCTCGACCTTTAAGAAAACACATAACTAGTGAA
TATAAAAAGTAGTGAAATTTCGAATGCTTAAGAGTAAAATAATTACCAAGACATTATCAA
ATGAGAATGTGAAGCATCAGTGATTCAGTGTGGTCTCCTTTCTAATATATTCATCACATT
TTTGCATATCACAAGTGTTCTTCAAACGTTAAAACTCAGAAACCAACATATCAATTTTAA
GCTGTAAAGAAACTGATGAAAACCAACTTACAGGATATATTCAATTGTAAATGGCTTCAT
GTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAANAAGAAAG
AGTAATTACCAATTTTTTTTCATTCAAAATGTAATT
   
Sequence Name 18-K118465-0022-015-E03C-8409
GenBank Accession LN494514 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>18-K118465-0022-015-E03C-8409
CCTTTACAATTGAAATATCCTGTAAGTTGGTTTTCATCAGTTTCTTTACAGCTTAAAATT
GATATGTTGGTTTCTGAGTTTTAACGTTTGAAGAACACTTGTGATATGCAAAAATGTGAT
GAATATATTAGAAAGGAGACCACACTGAATCACTGATGCTTCACATTCTCATTTGATAAT
GTCTTGGTAATTATTTTACTCTTAAGCATTCGAAATTTCACTACTTTTTATATTCACTAG
TTATGTGTTTTCTTAAAGGTCGAGGACATTGTGGATACTGGCAATACACTTAGCTGCCTG
ATTGAGCACATGAAAGCAAAAAAGGCGTCATCTGTTTCGGTTTGCACTCTCCTCGACAAG
CCATCGAGAAGAAAGGTTCATTATAAGCTGGTTGGAAAGGGGAAATTCTACAGTGGTTTT
GAAGTAAGCTGTTTCTTGTTTCTCTCTGTTCAATATATGTATTATCATTATACGTCTGTC
TAAGGCCTTTGGAATGTTTAAGCCACCTTAACCAGAGTATACAGCTATTTATAGAATAGG
TGAGACCATGACATTATGCTTTCCGTTCTTATTTGCCCTGTTATGCCTTTTCTTAGCTAA
ACCATGGTAAACAAAAGTATTTGTTGGTGTTAGGAACTAGCCTGAATTAAGAATATATAT
TTCATGGCTTTGTGTTGAACATGAGTTGTATCTTCTGAATATGTGCAGTGTCCAGATGAA
TTTGTCGTGGGCTATGGCATGGATTTTGCAGAACAATACCGCA

Last Updated on 06.12.2018 16:11