Sequences from confirmation amplicon


Confirmation sequences for line 139B11 and hit At1g71880 are shown.
Line ID 139B11
Locus At1g71880 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:27055747
Primers used for PCR RK15 - 8474, show primer details
   
Sequence Name 47-K015848-022-139-B11C-8409
GenBank Accession LN506027 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>47-K015848-022-139-B11C-8409
GTAGATTTCCCGGACATGAAGCCATTAATATATACATCTTTAATATTAAAGTTACTAAAG
TTACAAATATGTGGGACCGTAACTATTATATTTTGGTTTCATCCACCTTATGCAATTATA
ACATAGGTATTTAAATTGTTTTTGTGATGTTTTGATTTACAGGACTTTCATTAGGAGTTT
TAAATTTGGCGATTGTGATACCACAAATGATAGTCTCACTAGGAGGTGGACCTTTCGACG
CCTTGTTCGGCGGTGGAAACTTACCGGCGTTTATCGTTGCAGCAATTGCAGCGGCGATTA
GTGGAGTATTAGCGTTAACCGTTTTACCTTCACCACCACCGGACGC
   
Sequence Name 48-K015848-022-139-B11C-RK15
GenBank Accession LN506354 [GenBank]
Sequencing primer gene specific primer, RK15: 5'-TTTTATTTGGGTTTTTGCAGTTTC-3'
Sequence (A. th genome BLAST matches underlined) 
>48-K015848-022-139-B11C-rk15
CAGTAATACTGTATTTGATGAGTTTCATTAAAAAAAAAAAATAGTAAACTAGGGGAATCC
TCCCATGGTCGTTGCCTTGGGTGCGTCCGGGGGGGGTGAAGGTAAAACGGTTAACGCTAA
TACTCCACTAATCGCCGCTGCAATTGCTGCAACGATAAACGCCGGTAAGTTTCCCCCGCC
GAACAAGGGGTCGAAAGGTCCACCTCCTAGGGAGACTATCATTTGGGGTATCACAATCGC
CAAATTTAAAACTCCTAATGAAAGTCCTGTAAATCAAAACATCCCAAAAACAATTTAAAT
ACCTATGTTATAATTGCATAAGGGGGATGAAACCAAAATATAATAGTTACGGCCCCACAT
ATTTGTAACTTTAGTAACTTTAATATTAAAGATGTATATATTAATGGCTTCATGTCCGGG
AAATCTACATGGATCACCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATT
ACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCCGCGTTTTTATAAAATGAAAGTA
CTTTTTGATAAAACGACAAATTACGATCCGTCGTATTTATGGGCGAAAGCAATAAACAAA
TTATTCTAATTCGGAAATCTTTATTTCGA

Last Updated on 06.12.2018 16:11