Sequences from confirmation amplicon


Confirmation sequences for line 940H02 and hit At1g72970 are shown.
Line ID 940H02
Locus At1g72970 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:27453095
Primers used for PCR AJ09 - 8474, show primer details
   
Sequence Name 09-K105456-0022-940-H02C-AJ09
GenBank Accession LN490173 [GenBank]
Sequencing primer gene specific primer, AJ09: 5'-ACAGAATGGGACACACCTAAAATC-3'
Sequence (A. th genome BLAST matches underlined) 
>09-K105456-0022-940-H02C-AJ09
CCACATCTCTTTATACACAACTTGTGTTTTTTTTTATATATGATTTTTTTACCGAATGTT
GTTGTCTAACCAAAATTCAATGTACCGTATAAGACTATAAGTTATACAACTCGTAGAAAT
GGTTACATATATGTAACAATATATAGAGACACATGTGGGTGTAATTAATATAGTTGCTCT
GTTGGATCGTTAGTGTTATTTATAGGAGAAGAAAACACACAAAACAAGACCATTCATCAA
AAGCCACATAAGAAGTTGATGCAGAAGCAATAATAACAAAACAAAAAAACATTAAAAAAA
AAAGCTCTTCAGTTTTGGTTTCTGCAGTCTCCAATGGCTCTCAAGCTCTTTCTCTTTGCT
CTTCTTCTCTGTCTCCCGACTTCTCTCTCCTCCACTGCCTCTAAAGGTCACTTCTCTTTC
TCTCTTTAAACACAATCCTCCCATTACAAAGTTCTCTCTGTTTTTATCTTTCTCTTGGTC
GTTGATGATTCTGTCTTGTTTTTAGCTATAGCTCTGTTTTTCGAAACTAGTTACTTATTC
ATAGACATGATATACATGGCCAAAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGC
AATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATT
CAAAAATGTAAATGTCCG
   
Sequence Name 25-K105457-0022-940-H02C-8409
GenBank Accession LN497441 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>25-K105457-0022-940-H02C-8409
TTTTCGGCCATGTATATCATGTCTATGAATAAGTAACTAGTTTCGAAAAACAGAGCTATA
GCTAAAAACAAGACAGAATCATCAACGACCAAGAGAAAGATAAAAACAGAGAGAACTTTG
TAATGGGAGGATTGTGTTTAAAGAGAGAAAGAGAAGTGACCTTTAGAGGCAGTGGAGGAG
AGAGAAGTCGGGAGACAGAGAAGAAGAGCAAAGAGAAAGAGCTTGAGAGCCATTGGAGAC
TGCAGAAACCAAAACTGAAGAGCTTTTTTTTTTAATGTTTTTTTGTTTTGTTATTATTGC
TTCTGCATCAACTTCTTATGTGGCTTTTGATGAATGGTCTTGTTTTGTGTGTTTTCTTCT
CCTATAAATAACACTAACGATCCAACAGAGCAACTATATTAATTACACCCACATGTGTCT
CTATATATTGTTACATATATGTAACCATTTCTACGAGTTGTATAACTTATAGTCTTATAC
GGTACATTGAATTTTGGTTAGACAACAACATTCGGTAAAAAAATCATATAGTAAAAAAAA
ACACAAGTTGTGTAATAAAGAGATGTTGGGGTGGTCTACACTAAAACGTAAATTTGATTT
TAGGTGTGTCCCATTCT
Primers used for PCR 36wl - 8474, show primer details
   
Sequence Name 62-K118446-0022-940-H02C-36wl
GenBank Accession LN510855 [GenBank]
Sequencing primer gene specific primer, 36wl: 5'-AAAATTATACTTACGCAGCATCGG-3'
Sequence (A. th genome BLAST matches underlined) 
>62-K118446-0022-940-H02C-36wl
GGACCGCCCCGAGAACTCTAGCACGGGCGTTGTAAACGCCGTCAGTGGAAACAAACGCTT
GAGACGCGGAAGAAGCTGAAATGTCAGCAAGTCCGATGTGAAAATTCCTGAGGAAAGAAA
CGTTTGCGTTTGTAAACGGAACGCCACCTCTCTCTAAAACAAGAACGCTGAAATTCTGCG
ACAACGTTGCGGCGAGAGGACACCCTGCGGTTCCACCTCCGATGACTATGTAGTCGTAGG
ACGAATCTTGACCGTTGGATGAAAATGAAGATGAGGAAGATGATGAGAATGTGCTTGCTT
TATCGATGAATGTGTATCTGTATGGATTAAACTTCGACTTCTTCTCTTTACCTACAAAAT
CATTGTTTTTTTTAGTTGTACTATTCAATAAATAAAACAATTATGGGTAGTTGGTAGAAG
TAATTACACCATTAATGTGTTTATAGCAGAACATAACCCAAAAAACACACAACAGTTTAC
GCGTTAAAAAAAGACTGGACTGTGCACTAGTGTCCTGGTTACTCAAGATTCGGTCATGAT
GGATCAAGAAACAGAGTCTTTAATATATAAAACAGAAATCTACATGGATCAGCAATGAGT
ATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAAT
GTAGATGTCCGCAGCGGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTAC
GATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTAT
TTCGACGTGTCTACATTCACGTCCAAATGGGGGCTTAGATGAGAAACTTCACGATCGATG
CCTTGATTTC
   
Sequence Name 22-K118445-0022-940-H02C-8409
GenBank Accession LN496315 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>22-K118445-0022-940-H02C-8409
GCCATCGGAGGTGGAACCGCAGGGTGTCCTCTCGCCGCAACGTTGTCGCACAATTTCAGC
GTTCTTGTTTTAGAGAGAGGTGGCGTTCCGTTTACAAACGCAAACGTTTCTTTCCTCAGG
AATTTTCACATCGGACTTGCTGACATTTCAGCTTCTTCCGCGTCTCAAGCGTTTGTTTCC
ACTGACGGCGTTTACAACGCCCGTGCTAGAGTTCTCGGTGGCGGTTCCTGTATTAACGCC
GGTTTTTACTCCAGAGCCAATGCTGCGTAAGTATAATTTTAAACNCCNACGGTCAAGATT
CGTCCTACGACTACATAGTAATCGGAGGTGGAACCACAGGATGTCCTCTCGCCGCAACGT
TGTCGCAAAAT
Primers used for PCR AJ10 - 8474, show primer details
   
Sequence Name 34-K118466-0022-940-H02C-AJ10
GenBank Accession LN501599 [GenBank]
Sequencing primer gene specific primer, AJ10: 5'-GCTTGCTTTATCGATGAATGTGTA-3'
Sequence (A. th genome BLAST matches underlined) 
>34-K118466-0022-940-H02C-AJ10
TTTTTTTAGTCGTACTATTCAATAAATAAAACAATTATGGGTAGTTGGTAGAAGTAATTA
CACCATTAATGTGTTTATAGCAGAACATAACCCAAAAAACACACAACAGTTTACGCGTTA
AAAAAAGACTGGACTGTGCACTAGTGTCCTGGTTACTCAAGATTCGGTCATGATGGATCA
AGAAACAGAGTCTTTAATATATAAAACAGAAATCTACATGGATCAGCAATGAGTATGATG
GTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGAT
GTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGT
CGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATTTCGACG
TGTCTACATTCACGTCCAAATGGGGGCTTAGATGAGAAACTTCACGATCGATGCCTTGAT
TTCGTCCAAATTGTATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATC
AGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCA
ATTCAAAAATGTAGATGTCCG
   
Sequence Name 07-K118465-0022-940-H02C-8409
GenBank Accession LN489476 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>07-K118465-0022-940-H02C-8409
AATTAAAACTTGGTAATTACTCTTTCTTTTTCTCCACATGCACCATCATACTCATTGCTG
ATCCATGTAGATTTCTGTTTTATATATTAAAGACTCTGTTTCTTGATCCATCATGACCGA
ATCTTGAGTAACCAGGACACTAGTGCACAGTCCAGTCTTTTTTTAACGCGTAAACTGTTG
TGTGTTTTTTGGGTTATGTTCTGCTATAAACACATTAATGGTGTAATTACTTCTACCAAC
TACCCATAATTGTTTTATTTATTGAATAGTACAACTAATAAAAACAATGATTTTGTAGGT
CAAGAGAAGAAGTCGAAGTTTAATCCATACAGATACACATTCGTCGATAAAGCAAGCAAA

Last Updated on 06.12.2018 16:11