Sequences from confirmation amplicon


Confirmation sequences for line 436D09 and hit At1g73100 are shown.
Line ID 436D09
Locus At1g73100 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:27491233
Primers used for PCR JV46 - 8474, show primer details
   
Sequence Name 09-K101324-0022-436-D09C-8409
GenBank Accession LN490108 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>09-K101324-0022-436-D09C-8409
ATTTAAATTGAATAATCCTTTCATTTCTTTTTCTACATTTTATTTCACTCTCTCTCTCTC
GTCAGGAATCGTCTCTGCTCTTTCATCATCCTCCGGTCTCTCTCTCCCTCTCTCTAATTC
GGAGCGCTAATTTCTGTTTTATCATCTTCTTGAGGGTTCTTCTAGTTGCATAGCTAGGTC
TAAATAAAAAAGTAGGGATCTTGATGCAAAAAGGAAACTTTTTTTTCCTGGTTTCCCTAG
GCATGAAAGATCTGTTGGTGTTTAGAATTTGTGGGCTTCAATTACTTTGACTTCTAGGTT
GATGATTTTGTTACATTTTGGGGGAAGTAATCTATGAACGAAAAGCACAAACTGCTATGT
ATTCCTTACGAAGCGCTTTTAGATGCATTTAGGTTTTATGCAAAAAGTCCCATAACTTGT
TGTCTGATATCAAAGTTTTCATTTTGGGAAGTGAATG
   
Sequence Name 09-K101341-0022-436-D09C-JV46
GenBank Accession LN490109 [GenBank]
Sequencing primer gene specific primer, JV46: 5'-ACATTCACTTCCCAAAATGAAAAC-3'
Sequence (A. th genome BLAST matches underlined) 
>09-K101341-0022-436-D09C-JV46
GTTAGGGACTTTTTGCTAAACCTAAATGCATCTAAAAGCGCTTCGTAAGGAATACATAGC
AGTTTGTGCTTTTCGTTCATAGATTACTTCCCCCAAAATGTAACAAAATCATCAACCTAG
AAGTCAAAGTAATTGAAGCCCACAAATTCTAAACACCAACAGATCTTTCATGCCTAGGGA
AACCAGGAAAAAAAAGTTTCCTTTTTGCATCAAGATCCCTACTTTTTTATTTAGACCTAG
CTATGCAACTAGAAGAACCCTCAAGAAGATGATAAAACAGAAATTAGCGCTCCGAATTAG
AGAGAGGGAGAGAGAGACCGGAGGATGATGAAAGAGCAGAGACGATTCCTGACGAGAGAG
AGAGAGTGAAATAAAATGTAGAAAAAGAAATGAAAGGATATATTCAATTGTAAATGGCTT
CATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGA
AAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCACGGT

Last Updated on 06.12.2018 16:11