Sequences from confirmation amplicon


Confirmation sequences for line 051A10 and hit At1g73590 are shown.
Line ID 051A10
Locus At1g73590 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:27662013
Primers used for PCR KP01 - 8474, show primer details
   
Sequence Name 19-K021167-022-051-A10C-8409
GenBank Accession LN494573 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>19-K021167-022-051-A10C-8409
ATTTCCCGGATAAGAAAATAAAAAATGGTTCCTCCTATTCAAGGGAGAGACATGTTATGA
TGATAAGCAGAGAACTGTGGAGCATGCTTTCTGCTGTGAAGCCAGTTTTGTTTGCTTTTT
TGATACAAACATGTTTCAAAAAAACTTCAAAGAAAAAGAAAACAGAGAGAATCATGCTTC
CACATGATTTGTTTTTTTTCTTAAAATCATTTAAAATTTAAAAAACACCATAAAGAATGA
AGGTCAGAAATAAAGAGAACAATTGACTGTTTTATGGTATTTAATTATGTAATAAATGAA
AAGAGGTAAAAATAGAAGAAAAGAGAAAAAGGAACTGACCAAGACTGAACATAGCCATGC
CTAGACCTGCATCTGAGAGTATGGAGATAGACTTTGCTAT
   
Sequence Name 23-K021167-022-051-A10C-KP01
GenBank Accession LN496379 [GenBank]
Sequencing primer gene specific primer, KP01: 5'-ATGCCAGCTCTTATAGCAAAGTCT-3'
Sequence (A. th genome BLAST matches underlined) 
>23-K021167-022-051-A10C-KP01
TCCATACTCTCAGATGCAGGTCTAGGCATGGCTATGTTCAGTCTTGGTCAGTTCCTTTTT
CTCTTTTCTTTTATTTTTACCTCTTTTCATTTATTACATAATTAAATACCATAAAACAGT
CAATTGTTCTCTTTATTTCTGACCTTCATTCTTTATGGTGTTTTTTAAATTTTAAATGAT
TTTAAGAAAAAAAACAAATCATGTGGAAGCATGATTCTCTCTGTTTTCTTTTTCTTTGAA
GTTTTTTTGAAACATGTTTGTATCAAAAAAGCAAACAAAACTGGCTTCACAGCAGAAAGC
ATGCTCCACAGTTCTCTGCTTATCATCATAACATGTCTCTCCCTTGAATAGGAGGAACCA
TTTTTTATTTTCTTATCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATAT
TGAGAAA
Primers used for PCR FM09 - 8474, show primer details
   
Sequence Name 45-K029744-022-051-A10C-8409
GenBank Accession LN505415 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>45-K029744-022-051-A10C-8409
GCCATTTACAATTGAATATATATAGTTAATATATTTATATGTATTTGTTTATTCTTTTTG
CTGATCCAACAAACAATTTTTTTATACCTAATATTGACCCCCTTATCGATGAAAAAACTT
TCTTTTTTACCTTGTTTATGTTTCAGTAGACTTCCATCAACTGATTATAAAATTTGTGCT
TAATCATGACTTTATGGAATGAATAGTTTAGGATAACGATAATGATGATGTTAATGTTGT
GGTGGTGGATCAGTGGCTGTGATGATTATTTTTATGTCACTCTTTTGCAGCTAAAAACAG
TGTATAAACTTAAATTAACCAAAAAGCAAAACAATAATAAATTATTTGTTTAAATTTGTG
GCTTTTTTTGGATGTGTTAAAGGGTTGTTCATGGCGTTAAACCCAAGAATAATAGCTTGT
GGAACCGGAAGA
   
Sequence Name 46-K029744-022-051-A10C-FM09
GenBank Accession LN505759 [GenBank]
Sequencing primer gene specific primer, FM09: 5'-GCTCTTCTGTTTCCACAAGCTATT-3'
Sequence (A. th genome BLAST matches underlined) 
>46-K029744-022-051-A10C-FM09
CTTTACACATCCAAAAAAAGCCACAAATTTAAACAAATAATTTATTATTGTTTTGCTTTT
TGGTTAATTTAAGTTTATACACTGGTTTTTAGCTGCAAAAGAGTGACATAAAAATAATCA
TCACAGCCACTGATCCACCACCACAACATTAACATCATCATTATCGTTATCCTAAACTAT
TCATTCCATAAAGTCATGATTAAGCACAAATTTTATAATCAGTTGATGGAAGTCTACTGA
AACATAAACAAGGTAAAAAAGAAAGTTTTTTCATCGATAAGGGGGTCAATATTAGGTATA
AAAAAATTGTTTGTTGGATCAGCAAAAAGAATAAACAAATACATATAAATATATTAACTA
TATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTA
TGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATG
TAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGA
TCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATTT
CGACGTGTCTACATT

Last Updated on 06.12.2018 16:11