Sequences from confirmation amplicon


Confirmation sequences for line 800G02 and hit At1g73880 are shown.
Line ID 800G02
Locus At1g73880 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:27784926
Primers used for PCR 16dr - 8474, show primer details
   
Sequence Name 77-K110363-0022-800-G02C-16dr
GenBank Accession LN513565 [GenBank]
Sequencing primer gene specific primer, 16dr: 5'-AACCAAGGAAGAAATCAGATACGA-3'
Sequence (A. th genome BLAST matches underlined) 
>77-K110363-0022-800-G02C-16dr
GTATCCAGAGATAAGCGGCGCGTGGAGATTACCAAGCGCGTGGATCATTAAAGGGAAGCC
TGAAGGAGGTAAGTCTTGGACGTTTTCGACGCCGGAGGGGATTGAAGGGTGGGAGGGAAA
AGGGAGGATAAGTGGTTCGATGTTAACTACGGCGGAGAGAAGCGGAGAGAGAAAAGGAAG
GTTTTTTGGAGTGACTAGGACGGTTATTTTTAAGGCGGCGCCGCCGCGGAGAGCAAGGCG
GTGGGTGAAGTCGAGGAGGGGAATCATGTGACCTTGCGCCGGAAATGGGAAGATTAAGAC
ATGGGTCTTTGTCGGCTTGTTGTTTTCCTCGTTCACTTTCATAATGAGCTTTTCTAGTGG
GACTTTTTTTTGTTTATGCCGGAATGTTTTGGTGGAGCAAAGAGAAAAGAGTGATTTAAA
TAGATGTGTGTTAACTGTAAATGGCCGATTTGTGTACCCAATGTATTCCCAACACTTGGA
AAGAATGCTTACGACACTTTTGATAATACCGTGTGGTATTTTGATTAATG
   
Sequence Name 69-K110362-0022-800-G02C-8409
GenBank Accession LN512229 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>69-K110362-0022-800-G02C-8409
CATTTACAATTGAAAAGCTCTTCTCAAGTGTCATCTGACGCTGCATTAATCAAAATACCA
CACGGTATTATCAAAAGTGTCGTAAGCATTCTTTCCAAGTGTTGGGAATACATTGGGTAC
ACAAATCGGCCATTTACAGTTAACACACATCTATTTAAATCACTCTTTTCTCTTTGCTCC
ACCAAAACATTCCGGCATAAACAAAAAAAAGTCCCACTAGAAAAGCTCATTATGAAAGTG
AACGAGGAAAACAACAAGCCGACAAAGACCCATGTCTTAATCTTCCCATTTCCGGCGCAA
GGTCACATGATTCCCCTCCTCGACTTCACCCACCGCCTTGCTCTCCGCGGCGGCGCCGCC
TTAAAAATAACCGTCCTAGTCACTCCAAAAAACCTTCCTTTTCTCTCTCCGCTTCTCTCC
GCCGTAGTTAACATCGAACCACTTATCCTCCCTTTTCCCTCCCACCCTTCAATCCCCTCC
GGCGTCGAAAACGTCCAAGACTTACCTCCTTCAGGCTTCCCTTTAATGATCCACGCGCTT
GGTAATCTCCACGCGCCGCTTATCTCTTGGATTACTTCTCACCCTTCTCCTCCAGTAGCC
ATCGTATCTGATTTCTT

Last Updated on 06.12.2018 16:11